![Western Blot: Carbonic Anhydrase I/CA1 Antibody [NB120-6619] - Lane 1: Carbonic Anhydrase I. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase I antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP goat secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase I.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Western-Blot-NB120-6619-img0004.jpg "Western Blot: Carbonic Anhydrase I/CA1 Antibody [NB120-6619] - Lane 1: Carbonic Anhydrase I. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase I antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP goat secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase I.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-77740-0.1mg | |||
| HRP | NB600-942-0.1mg | |||
| Description | This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes] Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | Carbonic Anhydrase I/CA1/CA1 [Human Erythrocytes] (Uniprot: P00915) |
| Localization | Cytoplasmic |
| Specificity | Cross reactivity against Carbonic Anhydrase I/CA1 from other tissues and species may occur but have not been specifically determined. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | CA1 |
| Purity | Multi-step |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product has been tested in western blot and immunohistochemistry and is suitable in ELISA. Specific conditions for reactivity should be optimized by the end user. |
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| Control |
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| Storage | Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent) |
Secondary Antibodies |
Isotype Controls |
Research Areas for Carbonic Anhydrase I/CA1 Antibody (NB120-6619)Find related products by research area.
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![Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody [NB120-6619] - Tissue: spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Incubated at 5 ug/mL for 1 h at RT.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-I-CA1-Antibody-Immunohistochemistry-NB120-6619-img0001.jpg "Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody [NB120-6619] - Tissue: spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Incubated at 5 ug/mL for 1 h at RT.")
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