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Carbonic Anhydrase I/CA1 Antibody (1F1)

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Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Analysis of CA1 expression in transfected 293T cell line by CA1 monoclonal antibody (M10), clone 1F1. Lane 1: CA1 transfected lysatE (28.9 KDa). Lane 2: Non-transfected lysate.
Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Analysis of CA1 expression in transfected 293T cell line by CA1 monoclonal antibody (M10), clone 1F1. Lane 1: CA1 transfected lysatE (28.9 KDa). ...read more
Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Analysis of CA1 expression in human lung cancer.
Sandwich ELISA: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Detection limit for recombinant GST tagged CA1 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
1F1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Carbonic Anhydrase I/CA1 Antibody (1F1) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
CA1 (AAH27890, 1 a.a. ~ 261 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASPDWGYDDKNGPEQWSKLYPIANGNNQSPVDIKTSETKHDTSLKPISVSYNPATAKEIINVGHSFHVNFEDNDNRSVLKGGPFSDSYRLFQFHFHWGSTNEHGSEHTVDGVKYSAELHVAHWNSAKYSSLAEAASKADGLAVIGVLMKVGEANPKLQKVLDALQAIKTKGKRAPFTNFDPSTLLPSSLDFWTYPGSLTHPPLYESVTWIICKESISVSSEQLAQFRSLLSNVEGDNAVPMQHNNRPTQPLKGRTVRASF
Specificity
CA1 (1F1)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CA1
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against tissue lysate for WB. It has been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Carbonic Anhydrase I/CA1 Antibody (1F1)

  • CA1
  • CA-I
  • Car1
  • Carbonate dehydratase I
  • carbonic anhydrase 1
  • Carbonic anhydrase B
  • Carbonic Anhydrase I
  • carbonic anhydrase ICAB
  • carbonic dehydratase
  • EC 4.2.1.1

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CA1
Entrez
OMIM
Uniprot