![Western Blot: CaM Kinase II [p Thr306] Antibody [NBP2-60767] - specific immunolabelingof the ~50 kDa alpha and the ~60 kDa Beta CaM Kinase IIphosphorylated at Thr306 in the first lane (-). Phosphospecificity isshown in the second lane (+) where the immunolabeling iscompletely eliminated by lysate treatment with lambdaphosphatase (Lamba-Ptase, 800 units/1mg protein for 30 minutes).](http://images.novusbio.com/fullsize/CaM-Kinase-II-[p-Thr306]-Antibody-Western-Blot-NBP2-60767-img0001.jpg "Western Blot: CaM Kinase II [p Thr306] Antibody [NBP2-60767] - specific immunolabelingof the ~50 kDa alpha and the ~60 kDa Beta CaM Kinase IIphosphorylated at Thr306 in the first lane (-). Phosphospecificity isshown in the second lane (+) where the immunolabeling iscompletely eliminated by lysate treatment with lambdaphosphatase (Lamba-Ptase, 800 units/1mg protein for 30 minutes).")
| Reactivity | Hu, Mu, Rt, Bv, Ca, Ch, Pm, Sh, Xp, ZeSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding CaM Kinase II conjugated to KLH. Accession # P11310 |
| Modification | p Thr306 |
| Specificity | Specific for endogenous levels of the ~50 kDa alpha CaM Kinase II and the ~60 kDa beta CaM Kinase II proteins phosphorylated at Thr306. Immunolabeling is completely eliminated by treatment with lambda-phosphatase. It has been reported that this antibody may recognize some level of non-phosphorylated pure recombinant protein, but in our hands (as shown in our WB image) in native tissue, the antibody reacts in a phospho-specific manner. |
| Predicted Species | Zebrafish (100%), Bovine (100%), Chicken (100%), Canine (100%), Sheep (100%), Primate (100%), Xenopus (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CAMK2B |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB analysis shows bands at 50/60 kDa |
| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol |
| Preservative | No Preservative |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for CaM Kinase II Antibody (NBP2-60767)Find related products by research area.
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| Gene Symbol | CAMK2B |
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