![Western Blot: C1GALT1 Antibody (1F1) [H00056913-M01] - C1GALT1 monoclonal antibody (M01), clone 1F1 Analysis of C1GALT1 expression in HeLa.](http://images.novusbio.com/fullsize/C1GALT1-Antibody-1F1-Western-Blot-H00056913-M01-img0007.jpg "Western Blot: C1GALT1 Antibody (1F1) [H00056913-M01] - C1GALT1 monoclonal antibody (M01), clone 1F1 Analysis of C1GALT1 expression in HeLa.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clone | 1F1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | C1GALT1 (NP_064541, 264 a.a. ~ 363 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ETFHPFVPEHHLIKGYLPRTFWYWNYNYYPPVEGPGCCSDLAVSFHYVDSTTMYELEYLVYHLRPYGYLYRYQPTLPERILKEISQANKNEDTKVKLGNP |
| Specificity | C1GALT1 - core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1 |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | C1GALT1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
![Immunohistochemistry Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody [Unconjugated]](https://images.novusbio.com/images2/GCNT1_AF7248_Immunohistochemistry_22592.jpg)
![Immunocytochemistry Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody [Unconjugated]](https://images.novusbio.com/images2/GCNT1_AF7248_Immunocytochemistry_12684.jpg)
![Western Blot Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Antibody [Unconjugated]](https://images.novusbio.com/images2/GCNT1_AF7248_Western_Blot_12747.jpg)
| Publication using H00056913-M01 | Applications | Species |
|---|---|---|
| Argueso P, Guzman-Aranguez A, Mantelli F et al. Association of cell surface mucins with galectin-3 contributes to the ocular surface epithelial barrier. J Biol Chem 284(34):23037-45. 2009-08-21 [PMID: 19556244] |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: C1GALT1 Antibody (1F1) [H00056913-M01] - Analysis of monoclonal antibody to C1GALT1 on formalin-fixed paraffin-embedded human hepatocellular carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/C1GALT1-Antibody-1F1-Immunohistochemistry-Paraffin-H00056913-M01-img0005.jpg "Immunohistochemistry-Paraffin: C1GALT1 Antibody (1F1) [H00056913-M01] - Analysis of monoclonal antibody to C1GALT1 on formalin-fixed paraffin-embedded human hepatocellular carcinoma. Antibody concentration 3 ug/ml.")
![ELISA: C1GALT1 Antibody (1F1) [H00056913-M01] - Detection limit for recombinant GST tagged C1GALT1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/C1GALT1-Antibody-1F1-Sandwich-ELISA-H00056913-M01-img0006.jpg "ELISA: C1GALT1 Antibody (1F1) [H00056913-M01] - Detection limit for recombinant GST tagged C1GALT1 is approximately 0.3ng/ml as a capture antibody.")
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