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c-Myb Antibody

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Western Blot: c-Myb Antibody [NBP1-52040] - (0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

c-Myb Antibody Summary

Immunogen
internal region
Epitope
C-HRDQTVKTQKENT
Specificity
This antibody is expected to recognize all reported isofoms. (NP_001123645.1; NP_005366.2; NP_001123644.1; NP_001155128.1; NP_001155129.1; NP_001155130.1; NP_001155131.1; NP_001155132.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MYB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA detection limit 1:8000
  • Western Blot
Application Notes
This antibody works in ELISA and Western Blot. Approx 85kDa band observed in lysates of cell line Jurkat (calculated MW of 85.5kDa according to NP_001123645.1). Recommended concentration: 0.5-1.5ug/ml.
Publications
Read Publication using NBP1-52040.

Reactivity Notes

Human, Mouse, Rat, Dog, Cow, Pig

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for c-Myb Antibody

  • c-myb protein (140 AA)
  • Cmyb
  • c-myb
  • c-myb_CDS
  • c-myb10A_CDS
  • c-myb13A_CDS
  • c-myb14A_CDS
  • c-myb8B_CDS
  • efg
  • Proto-oncogene c-Myb
  • transcriptional activator Myb
  • v-myb avian myeloblastosis viral oncogene homolog
  • v-myb myeloblastosis viral oncogene homolog (avian)

Background

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for c-Myb Antibody (NBP1-52040)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYB
Entrez
Uniprot