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c-Myb Antibody

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Western Blot: c-Myb Antibody [NB100-61662] - Staining of MOLT4 cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.
Western Blot: c-Myb Antibody [NB100-61662] - Staining of MOLT4 cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: c-Myb Antibody [NB100-61662] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Immunohistochemistry-Paraffin: c-Myb Antibody [NB100-61662] - Staining of paraffin embedded Human Kidney. Antibody at 4 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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c-Myb Antibody Summary

Immunogen
Peptide with sequence C-QRHYNDEDPEKEKR corresponding to internal region according to NP_001123645.1, NP_005366.2, NP_001123644.1, NP_001155128.1, NP_001155129.1, NP_001155130.1, NP_001155131.1.
Epitope
QRHYNDEDPEKEKR
Specificity
This antibody is expected to recognize isoforms 1-7 only (Numbering from NCBI RefSeq). (NP_001123645.1; NP_005366.2; NP_001123644.1; NP_001155128.1; NP_001155129.1; NP_001155130.1; NP_001155131.1)
Predicted Species
Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MYB
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 4 ug/mL
  • Immunohistochemistry-Paraffin 4 ug/mL
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1 - 3 ug/mL
Application Notes
Western blot: Approx 70 kDa band observed in lysates of cell line MOLT4 and in preliminary testing of Human Bone Marrow and Lymph Node lysate (calculated MW of 72.3kDa according to NP_005366.2).
Control
c-Myb Overexpression Lysate
Publications
Read Publication using NB100-61662.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for c-Myb Antibody

  • c-myb protein (140 AA)
  • Cmyb
  • c-myb
  • c-myb_CDS
  • c-myb10A_CDS
  • c-myb13A_CDS
  • c-myb14A_CDS
  • c-myb8B_CDS
  • efg
  • Proto-oncogene c-Myb
  • transcriptional activator Myb
  • v-myb avian myeloblastosis viral oncogene homolog
  • v-myb myeloblastosis viral oncogene homolog (avian)

Background

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for c-Myb Antibody (NB100-61662)(1)


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Product General Protocols

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Video Protocols

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Control Lysate(s)

c-Myb Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYB
Entrez
Uniprot