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BTK [p Tyr551] Antibody (797837) [Unconjugated]

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Western blot shows lysates of RL human non-Hodgkin's lymphoma B cell line and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 0.1 mM Pervanadate (PV) for 10 minutes and 12 µg/mL Goat Anti-Human IgM µ Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # G-105-C) for 2 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-BTK (Y551) Monoclonal Antibody (Catalog # MAB7659) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-BTK (Y551) at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of RL human non-Hodgkin's lymphoma B cell line and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 0.1 mM Pervanadate (PV) for 10 minutes and 12 µg/mL Goat ...read more
Simple Western lane view shows lysates of Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with ultraviolet light (UV), loaded at 0.2 mg/mL. A specific band was detected for Phospho-BTK (Y551) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clone
797837
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7659-SP
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BTK [p Tyr551] Antibody (797837) [Unconjugated] Summary

Immunogen
Phosphopeptide containing the human BTK Y551 site
Modification
p Tyr551
Specificity
Detects human BTK when phosphorylated at Y551.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
BTK
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
MAB7659 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BTK [p Tyr551] Antibody (797837) [Unconjugated]

  • Agammaglobulinaemia tyrosine kinase
  • AGMX1
  • AGMX1MGC126262
  • AT
  • ATKIMD1
  • B-cell progenitor kinase
  • BPK
  • Bruton agammaglobulinemia tyrosine kinase
  • Bruton tyrosine kinase
  • BTK
  • dominant-negative kinase-deficient Brutons tyrosine kinase
  • EC 2.7.10
  • EC 2.7.10.2
  • IMD1
  • PSCTK1
  • tyrosine-protein kinase BTK
  • XLA
  • XLAMGC126261

Background

Bruton tyrosine kinase (Btk) is a 75 kDa cytoplasmic protein tyrosine kinase that is widely expressed in hematopoietic cells. Btk is required for B cell receptor signaling and B cell development. Defects in Btk result in X-linked agammaglobulinemia which is characterized by a severely decreased level of circulating antibodies. Like other Tec family kinases, Btk contains a Pleckstrin homology domain, a Tec homology domain, an SH3 domain, an SH2 domain, and a protein kinase domain. Association of the Btk SH2 domain with the B cell linker protein (BLNK) is required for the activation of PLCg by Btk. Btk can be activated by Lyn or Syk phosphorylation of Tyr551 within the catalytic domain. Within aa 546 - 556, human Btk shares 100% aa sequence identity with mouse and rat Btk.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BTK Antibody (MAB7659)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Western Blot.


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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol BTK