![Western Blot: BRPF1 Antibody [NBP2-15620] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [N1N2], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301f) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BRPF1-Antibody-Western-Blot-NBP2-15620-img0004.jpg "Western Blot: BRPF1 Antibody [NBP2-15620] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [N1N2], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301f) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Bv, ChSpecies Glossary |
| Applications | WB, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human BRPF1. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Rat (96%), Chicken (91%), Bovine (99%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BRPF1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 137 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: BRPF1 Antibody [NBP2-15620] - Mouse testis. Peregrin stained by Peregrin antibody [N1N2], N-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/BRPF1-Antibody-Immunohistochemistry-Paraffin-NBP2-15620-img0005.jpg "Immunohistochemistry-Paraffin: BRPF1 Antibody [NBP2-15620] - Mouse testis. Peregrin stained by Peregrin antibody [N1N2], N-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: BRPF1 Antibody [NBP2-15620] - Western Blot analysis of BRPF1 expression in transfected 293T cell line by BRPF1 polyclonal antibody. A: Non-transfected lysate. B: BRPF1 transfected lysate. 7. 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/BRPF1-Antibody-Western-Blot-NBP2-15620-img0001.jpg "Western Blot: BRPF1 Antibody [NBP2-15620] - Western Blot analysis of BRPF1 expression in transfected 293T cell line by BRPF1 polyclonal antibody. A: Non-transfected lysate. B: BRPF1 transfected lysate. 7. 5% SDS PAGE gel, diluted at 1:500.")
![Knockout Validated: BRPF1 Antibody [NBP2-15620] - Wild-type (WT) and Peregrin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRPF1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BRPF1-Antibody-Western-Blot-NBP2-15620-img0002.jpg "Knockout Validated: BRPF1 Antibody [NBP2-15620] - Wild-type (WT) and Peregrin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRPF1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: BRPF1 Antibody [NBP2-15620] - Wild-type (WT) and Peregrin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [N1N2], N-term diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15620_rabbit-polyclonal-brpf1-antibody-1610202419405746.jpg "Western Blot: BRPF1 Antibody [NBP2-15620] - Wild-type (WT) and Peregrin knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [N1N2], N-term diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry ASCL1/Mash1 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af2567_mouse-ascl1-mash1-affinity-purified-polyclonal-ab-immunohistochemistry-1732021122114.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")