![Western Blot: BRPF1 Antibody (HL2064) - Azide and BSA Free [NBP3-25595] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [HL2064] (NBP3-25595) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25595_rabbit-brpf1-mab-hl2064-azide-and-bsa-free-2120249393579.jpg "Western Blot: BRPF1 Antibody (HL2064) - Azide and BSA Free [NBP3-25595] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [HL2064] (NBP3-25595) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC |
| Clone | HL2064 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of human BRPF1 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | BRPF1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BRPF1 |
![Western Blot: BRPF1 Antibody (HL2064) - Azide and BSA Free [NBP3-25595] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [HL2064] (NBP3-25595) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25595_rabbit-brpf1-mab-hl2064-azide-and-bsa-free-212024940388.jpg "Western Blot: BRPF1 Antibody (HL2064) - Azide and BSA Free [NBP3-25595] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [HL2064] (NBP3-25595) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Knockout Validated: BRPF1 Antibody (HL2064) - Azide and BSA Free [NBP3-25595] - Wild-type (WT) and BRPF1 knockout (KO) 293T cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [HL2064] (NBP3-25595) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/nbp3-25595_rabbit-brpf1-mab-hl2064-azide-and-bsa-free-2120249415988.jpg "Knockout Validated: BRPF1 Antibody (HL2064) - Azide and BSA Free [NBP3-25595] - Wild-type (WT) and BRPF1 knockout (KO) 293T cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Peregrin antibody [HL2064] (NBP3-25595) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.")
![Immunohistochemistry-Paraffin: BRPF1 Antibody (HL2064) - Azide and BSA Free [NBP3-25595] - Peregrin antibody [HL2064] detects Peregrin protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Peregrin stained by Peregrin antibody [HL2064] (NBP3-25595) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25595_rabbit-brpf1-mab-hl2064-azide-and-bsa-free-2120249371577.jpg "Immunohistochemistry-Paraffin: BRPF1 Antibody (HL2064) - Azide and BSA Free [NBP3-25595] - Peregrin antibody [HL2064] detects Peregrin protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Peregrin stained by Peregrin antibody [HL2064] (NBP3-25595) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry ASCL1/Mash1 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af2567_mouse-ascl1-mash1-affinity-purified-polyclonal-ab-immunohistochemistry-1732021122114.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")