![Western Blot: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of BRG1 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Western-Blot-NBP2-67720-img0013.jpg "Western Blot: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of BRG1 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | SN20-03 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within human Brg1 aa 240-280. (SwissProt: P51532 Human; SwissProt: Q3TKT4 Mouse; SwissProt: Q8K1P7 Rat) |
| Localization | Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SMARCA4 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Brg1 Antibody (NBP2-67720)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SMARCA4 |
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720] - Staining BRG1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunocytochemistry-Immunofluorescence-NBP2-67720-img0010.jpg "Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720] - Staining BRG1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse epididymis tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0019.jpg "Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse epididymis tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of BRG1 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Western-Blot-NBP2-67720-img0011.jpg "Western Blot: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of BRG1 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720] - Staining BRG1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunocytochemistry-Immunofluorescence-NBP2-67720-img0001.jpg "Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720] - Staining BRG1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720] - Staining BRG1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunocytochemistry-Immunofluorescence-NBP2-67720-img0002.jpg "Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720] - Staining BRG1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720] - Staining BRG1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunocytochemistry-Immunofluorescence-NBP2-67720-img0009.jpg "Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-67720] - Staining BRG1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0003.jpg "Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-BRG1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human kidney tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0004.jpg "Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human kidney tissue using anti-BRG1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human tonsil tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0005.jpg "Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human tonsil tissue using anti-BRG1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse colon tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0006.jpg "Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse colon tissue using anti-BRG1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse testis tissue using anti-BRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0007.jpg "Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse testis tissue using anti-BRG1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Brg1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0012.jpg "Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Brg1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0014.jpg "Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0015.jpg "Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0016.jpg "Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0017.jpg "Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Brg1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse kidney tissue using anti-Brg1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 2 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Brg1-Antibody-SN20-03-Immunohistochemistry-Paraffin-NBP2-67720-img0018.jpg "Immunohistochemistry-Paraffin: Brg1 Antibody (SN20-03) [NBP2-67720] - Analysis of paraffin-embedded mouse kidney tissue using anti-Brg1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 2 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot CBP/KAT3A Antibody (257003) [Unconjugated]](https://images.novusbio.com/images2/CBP_MAB2676_Western_Blot_6043.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")