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Brachyury Antibody (1161B) [Unconjugated]

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Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Rabbit Anti-Human Brachyury Monoclonal Antibody (Catalog # MAB20851) at a 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Rabbit Anti-Human Brachyury Monoclonal Antibody (Catalog # MAB20851) at a 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
1161B
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB20851-SP
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Brachyury Antibody (1161B) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
E. coli-derived recombinant human Brachyury
Met230-Met435
Accession # O15178
Specificity
Detects human Brachyury in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
T
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB20851 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Brachyury Antibody (1161B) [Unconjugated]

  • brachyury protein
  • Brachyury
  • MGC104817
  • Protein T
  • SAVA
  • T brachyury (mouse) homolog
  • T brachyury homolog
  • T Brachyury Transcription Factor
  • T, brachyury homolog (mouse)
  • T-Box Transcription Factor T
  • TBXT
  • TF
  • TFT

Background

Brachyury is a 435 aa T-box family transcription factor.  Human Brachyury shares 90% and 91% aa identity with mouse and rat Brachyury, respectively.  It is required in the early determination and differentiation of mesoderm.  Additionally, expression of Brachyury has been shown to be upregulated in a number of cancers, and in some cases, correlates with increased aggressiveness.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Brachyury Antibody (MAB20851)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ICC, ICC/IF, IHC, IHC-P, Immunohistochemistry, Western Blot.


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Showing Publications 1 - 10 of 10.
Publications using MAB20851 Applications Species
Thiyagarajan, R;Taub, M; Studies with Human-Induced Pluripotent Stem Cells Reveal That CTNS Mutations Can Alter Renal Proximal Tubule Differentiation International journal of molecular sciences 2023-11-30 [PMID: 38069326] (Immunohistochemistry, Human) Immunohistochemistry Human
Hilansi Rawat, Jessica Kornherr, Dorota Zawada, Sara Bakhshiyeva, Christian Kupatt, Karl-Ludwig Laugwitz et al. Recapitulating porcine cardiac development in vitro: from expanded potential stem cell to embryo culture models Frontiers in Cell and Developmental Biology 5/15/2023 [PMID: 37261075]
AM Drozd, L Mariani, X Guo, V Goitea, NA Menezes, E Ferretti Progesterone Receptor Modulates Extraembryonic Mesoderm and Cardiac Progenitor Specification during Mouse Gastrulation International Journal of Molecular Sciences, 2022-09-07;23(18):. 2022-09-07 [PMID: 36142249] (ICC/IF, Mouse) ICC/IF Mouse
H Tomita, KM Hines, JM Herron, A Li, DW Baggett, L Xu 7-Dehydrocholesterol-derived oxysterols cause neurogenic defects in Smith-Lemli-Opitz syndrome Elife, 2022-09-16;11(0):. 2022-09-16 [PMID: 36111785] (IHC, Mouse) IHC Mouse
RR Khadim, RK Vadivelu, T Utami, FG Torizal, M Nishikawa, Y Sakai Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs International Journal of Molecular Sciences, 2022-06-30;23(13):. 2022-06-30 [PMID: 35806277] (ICC, Human) ICC Human
Geetika Sahni, Shu‐Yung Chang, Jeremy Teo Choon Meng, Jerome Zu Yao Tan, Jean Jacques Clement Fatien, Carine Bonnard et al. A Micropatterned Human‐Specific Neuroepithelial Tissue for Modeling Gene and Drug‐Induced Neurodevelopmental Defects Advanced Science 2021-03-01 [PMID: 33717833]
Cong Wang, Shenghui Liang, Shijia Xing, Ke Xu, Huijie Xiao, Haiyue Deng et al. Endoplasmic Reticulum Stress Activation in Alport Syndrome Varies Between Genotype and Cell Type Frontiers in Genetics 2020-02-10 [PMID: 32117450]
Q Wang, A Vossen, Y Ikeda, P Devaux Measles vector as a multiple genes delivery platform facilitating iPSC reprogramming Gene Ther., 2019-02-04;0(0):. 2/4/2019 [PMID: 30718755]
A Yoney, F Etoc, A Ruzo, T Carroll, JJ Metzger, I Martyn, S Li, C Kirst, ED Siggia, AH Brivanlou WNT signaling memory is required for ACTIVIN to function as a morphogen in human gastruloids Elife, 2018-10-12;7(0):. 10/12/2018 [PMID: 30311909]
Lee D, Zhang Y, Kassam A, Park M, Gardner P, Prevedello D, Henry S, Horbinski C, Beumer J, Tawbi H, Williams B, Shaffrey M, Egorin M, Abounader R, Park D Combined PDGFR and HDAC Inhibition Overcomes PTEN Disruption in Chordoma. PLoS ONE, 2015-08-06;10(8):e0134426. 2015-08-06 [PMID: 26247786] (IHC-P, Western Blot, Human) IHC-P, Western Blot Human

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Bioinformatics

Gene Symbol T
Uniprot