Novus Biologicals products are now on bio-techne.com

Borealin Antibody (OTI4G3) - Azide and BSA Free

Images

 
Western Blot: Borealin Antibody (OTI4G3) - Azide and BSA Free [NBP2-71988] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Borealin.
Immunohistochemistry: Borealin Antibody (OTI4G3) - Azide and BSA Free [NBP2-71988] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Borealin Antibody (OTI4G3) - Azide and BSA Free [NBP2-71988] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for ...read more
Immunohistochemistry: Borealin Antibody (OTI4G3) - Azide and BSA Free [NBP2-71988] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for ...read more
Immunohistochemistry: Borealin Antibody (OTI4G3) - Azide and BSA Free [NBP2-71988] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Borealin Antibody (OTI4G3) - Azide and BSA Free [NBP2-71988] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
OTI4G3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Borealin Antibody (OTI4G3) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human CDCA8 (NP_060571) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CDCA8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Borealin Antibody (OTI4G3) - Azide and BSA Free

  • BOR
  • BOREALIN
  • cell division cycle associated 8
  • Cell division cycle-associated protein 8
  • Dasra B
  • DasraB
  • dasra-B
  • FLJ10468
  • FLJ12042
  • hDasra-B
  • MESRGP
  • PESCRG3
  • Pluripotent embryonic stem cell-related gene 3 protein

Background

Borealin (CDCA8) is a component of the chromosomal passenger complex (CPC), which is required for stability of the bipolar mitotic spindle and acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA.

Borealin has been shown to interact with Aurora B and survivin (BIRC5). Knockdown of Borealin by small interfering RNA resulted in severe chromosome misalignment at metaphase and accumulation of multiple interphase nuclei. Borealin is also related to the proliferation of human embryonic stem (hES) cells and is highly expressed in mouse undifferentiated ES cells.
Borealin antibodies are useful tools for stem cell studies and cell division research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-37471
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NB500-201
Species: Ca, Fe, Gp, Ha, Hu, Mu, Rt
Applications: ChIP, Dual ISH-IHC, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, Simple Western, WB
NB100-294
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, MiAr, WB
NB500-201
Species: Ca, Fe, Gp, Ha, Hu, Mu, Rt
Applications: ChIP, Dual ISH-IHC, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, Simple Western, WB
NBP2-87382
Species: Hu
Applications: IHC, IHC-P, WB
NB100-294
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, MiAr, WB
NBP1-84264
Species: Hu, Mu
Applications: ChIP, ICC/IF, IHC, IHC-P, KD, WB
NBP1-85729
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MAB6028
Species: Hu
Applications: WB
NB500-171
Species: Hu, I, Mu, Rt, Xp, Ye
Applications: ChIP, IB, ICC/IF, IHC, IHC-P, Single-Cell Western, WB
NB120-2938
Species: Bv, Hu, Mu
Applications: IHC, IHC-P, WB
H00011004-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, KD, S-ELISA, WB
NBP3-13389
Species: Hu
Applications: IHC, IHC-P, WB
NB100-1103
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
AF755
Species: Mu
Applications: Block, WB
NBP2-20383
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-02623
Species: Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB100-353
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC/IF, IP, WB
NB100-60454
Species: Hu
Applications: IP, WB (-)
NBP2-71988
Species: Hu
Applications: WB, IHC

Publications for Borealin Antibody (NBP2-71988) (0)

There are no publications for Borealin Antibody (NBP2-71988).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Borealin Antibody (NBP2-71988) (0)

There are no reviews for Borealin Antibody (NBP2-71988). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Borealin Antibody (NBP2-71988) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Borealin Antibody (OTI4G3) - Azide and BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol CDCA8