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BMI-1 Antibody (384509)

 (4 Publications)
 (1 Review)
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BMI‑1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human BMI‑1 Monoclonal Antibody (Catalog # MAB33342) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
BMI‑1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human BMI‑1 Monoclonal Antibody (Catalog # MAB33342) at 25 µg/mL overnight at 4 °C. Tissue was ...read more
BMI-1 was detected in immersion fixed paraffin-embedded sections of normal human breast using Human BMI-1 Monoclonal Antibody (Catalog # MAB33342) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
384509
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB33342-SP
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BMI-1 Antibody (384509) Summary

Immunogen
E. coli-derived recombinant human BMI-1
Asp96-Gly326
Accession # P35226
Specificity
Detects human BMI-1 in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
BMI1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB33342 in the following application:
Publications
Read Publications using
MAB33342 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMI-1 Antibody (384509)

  • B lymphoma Mo-MLV insertion region 1 homolog (mouse)
  • B lymphoma Mo-MLV insertion region 1 homolog
  • BMI1 polycomb ring finger oncogene
  • BMI1
  • BMI-1
  • FLVI2/BMI1
  • MGC12685
  • murine leukemia viral (bmi-1) oncogene homolog
  • PCGF4
  • polycomb complex protein BMI-1
  • polycomb group protein Bmi1
  • polycomb group ring finger 4
  • RING finger protein 51
  • RNF51
  • RNF51Polycomb group RING finger protein 4

Background

BMI-1 (B cell-specific Moloney-MLV integration site #1) is a 45 kDa protooncogene that is a class II member of the Polycomb group of genes. It participates in the formation of a large multimeric complex termed PRC1 that inhibits target gene transcription. Loss of BMI-1 function precludes stem cells from self-replicating. Human BMI-1 contains an N-terminal RING-finger domain (aa 17-56), an NLS (aa 81-95) and a C-terminal Pro/Ser-rich region (aa 251-326). Human BMI-1 is 99%, 97%, 99% and 99% aa identical to bovine, mouse, feline and canine BMI-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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BMI-1 Antibody
(384509)
MAB33342
BMI-1 Antibody (384509)
Species: Hu
Applications: IHC

Publications for BMI-1 Antibody (MAB33342)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IHC, IHC-P, Western Blot.


Filter By Application
IHC
(2)
IHC-P
(1)
Western Blot
(1)
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Human
(3)
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Review for BMI-1 Antibody (MAB33342) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB33342:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 IHC-P Human 02/20/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22730165
SpeciesHuman

FAQs for BMI-1 Antibody (MAB33342) (0)

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Secondary Antibodies

 

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Recent Reviews

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1
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 Verified Customer
02/20/2015
Application: IHC-P
Species: Human
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Bioinformatics

Gene Symbol BMI1
Uniprot