![Western Blot: BMAL1 Overexpression Lysate (Adult Normal) [NBL1-07723] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BMAL1.](http://images.novusbio.com/fullsize/BMAL1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07723-img0002.jpg "Western Blot: BMAL1 Overexpression Lysate (Adult Normal) [NBL1-07723] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BMAL1.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Description | BMAL1 Transient Overexpression LysateExpression Host: HEK293T Plasmid: RC217474 Accession#: NM_001030272 Protein Tag: C-MYC/DDK You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT. |
| Gene | ARNTL |
| Dilutions |
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| Application Notes | This product is intended for use as a positive control in Western Blot. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading. |
| Theoretical MW | 68.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | RIPA buffer |
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| Gene Symbol | ARNTL |
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![Flow (Intracellular): BMAL1 Antibody [NB100-2288] - An intracellular stain was performed on HeLa cells with NB100-2288AF647(blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](https://images.novusbio.com/images/BMAL1-Antibody-Flow-Intracellular-NB100-2288-img0017.jpg "Flow (Intracellular): BMAL1 Antibody [NB100-2288] - An intracellular stain was performed on HeLa cells with NB100-2288AF647(blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")