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BMAL1 Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Am, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, ChIP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-2288AF350
Alexa Fluor 405
NB100-2288AF405
Alexa Fluor 488
NB100-2288AF488
Alexa Fluor 532
NB100-2288AF532
Alexa Fluor 594
NB100-2288AF594
Alexa Fluor 647
NB100-2288AF647
Alexa Fluor 700
NB100-2288AF700
Alexa Fluor 750
NB100-2288AF750
Allophycocyanin
NB100-2288APC
Biotin
NB100-2288B
DyLight 350
NB100-2288UV
DyLight 405
NB100-2288V
DyLight 488
NB100-2288G
DyLight 550
NB100-2288R
DyLight 594
NB100-2288DL594
DyLight 650
NB100-2288C
DyLight 680
NB100-2288FR
DyLight 755
NB100-2288IR
FITC
NB100-2288F
HRP
NB100-2288H
Janelia Fluor 525
NB100-2288JF525
Janelia Fluor 549
NB100-2288JF549
Janelia Fluor 585
NB100-2288JF585
Janelia Fluor 635
NB100-2288JF635
Janelia Fluor 646
NB100-2288JF646
Janelia Fluor 669
NB100-2288JF669
PE
NB100-2288PE
PerCP
NB100-2288PCP
Unconjugated
NB100-2288
mFluor Violet 450 SE
NB100-2288MFV450
mFluor Violet 500 SE
NB100-2288MFV500
mFluor Violet 610 SE
NB100-2288MFV610
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BMAL1 Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Bacterially expressed human BMAL1 (amino acids 392-626). [UniProt# O00327].
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BMAL1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32732906). Amphibian reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for BMAL1 Antibody [CoraFluor™ 1]

  • ARNTL
  • aryl hydrocarbon receptor nuclear translocator-like protein 1
  • aryl hydrocarbon receptor nuclear translocator-like
  • basic-helix-loop-helix-PAS orphan MOP3
  • BHLHE5
  • bHLHe5brain and muscle
  • bHLH-PAS protein JAP3
  • BMAL1
  • BMAL1c
  • BMAL1TIC
  • Brain and muscle ARNT-like 1
  • Class E basic helix-loop-helix protein 5
  • JAP3
  • Member of PAS protein 3
  • member of PAS superfamily 3
  • MOP3
  • MOP3BMAL1c
  • PAS domain-containing protein 3
  • PASD3
  • PASD3MGC47515
  • TIC

Background

Aryl hydrocarbon receptor nuclear translocator-like (ARNTL), also known as Bmal1 or Mop3 is a major component of the circadian clock oscillator. Specifically, BMAL-1 acts as a general dimerization partner for a subset of the bHLH-PAS (basic-helix-loop-helix-PER-ARNT-SIM) superfamily of transcription regulators, is involved in circadian rhythm generation. BMAL 1 interacts with MOP4, CLOCK, Hypoxia-inducible factor-1 alpha and -2 alpha. This interaction appears to be distinct from that of the more characterized general partner, ARNT (HIF-1Beta).

BMAL1 is highly expressed in the adult brain, skeletal muscle and heart, and has been associated with hypertension and type 2 diabetes. Because of its role in the circadian clock oscillator, BMAL-1 is also thought to be invloven seasonal affective disorder. Therefore, BMAL1 antibodies are useful for circadian studies as well as heart and brain research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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BMAL1 Antibody
[CoraFluor™ 1]
NB100-2288CL1
BMAL1 Antibody [CoraFluor™ 1]
Species: Hu, Mu, Rt, Am, Pm
Applications: WB, Flow, ICC/IF, IHC, ChIP, KO

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Bioinformatics

Gene Symbol BMAL1