![Western Blot: BCAS2 Antibody (1D10) [H00010286-M01] - BCAS2 monoclonal antibody (M01), clone 1D10 Analysis of BCAS2 expression in K-562.](http://images.novusbio.com/fullsize/BCAS2-Antibody-1D10-Western-Blot-H00010286-M01-img0007.jpg "Western Blot: BCAS2 Antibody (1D10) [H00010286-M01] - BCAS2 monoclonal antibody (M01), clone 1D10 Analysis of BCAS2 expression in K-562.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, KD |
| Clone | 1D10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | BCAS2 (NP_005863, 116 a.a. ~ 225 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HQAVRIENLELMSQHGCNAWKVYNENLVHMIEHAQKELQKLRKHIQDLNWQRKNMQLTAGSKLREMESNWVSLVSKNYEIERTIVQLENEIYQIKQQHGEANKENIRQDF |
| Specificity | BCAS2 - breast carcinoma amplified sequence 2 |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BCAS2 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
| Publication using H00010286-M01 | Applications | Species |
|---|---|---|
| Grau L, Luque-Garcia JL, Gonzalez-Peramato P et al. A quantitative proteomic analysis uncovers the relevance of CUL3 in bladder cancer aggressiveness. PLoS One. 2013-01-01 [PMID: 23308193] (Human) | Human |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: BCAS2 Antibody (1D10) [H00010286-M01] - Analysis of BCAS2 over-expressed 293 cell line, cotransfected with BCAS2 Validated Chimera RNAi ( Cat # H00010286-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with BCAS2 monoclonal antibody (M01), clone 1D10 (Cat # H00010286-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/BCAS2-Antibody-1D10-Western-Blot-H00010286-M01-img0005.jpg "Western Blot: BCAS2 Antibody (1D10) [H00010286-M01] - Analysis of BCAS2 over-expressed 293 cell line, cotransfected with BCAS2 Validated Chimera RNAi ( Cat # H00010286-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with BCAS2 monoclonal antibody (M01), clone 1D10 (Cat # H00010286-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Western Blot: BCAS2 Antibody (1D10) [H00010286-M01] - Analysis of BCAS2 expression in transfected 293T cell line by BCAS2 monoclonal antibody (M01), clone 1D10.Lane 1: BCAS2 transfected lysate(26.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BCAS2-Antibody-1D10-Western-Blot-H00010286-M01-img0008.jpg "Western Blot: BCAS2 Antibody (1D10) [H00010286-M01] - Analysis of BCAS2 expression in transfected 293T cell line by BCAS2 monoclonal antibody (M01), clone 1D10.Lane 1: BCAS2 transfected lysate(26.1 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: BCAS2 Antibody (1D10) [H00010286-M01] - Detection limit for recombinant GST tagged BCAS2 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/BCAS2-Antibody-1D10-Sandwich-ELISA-H00010286-M01-img0006.jpg "Sandwich ELISA: BCAS2 Antibody (1D10) [H00010286-M01] - Detection limit for recombinant GST tagged BCAS2 is approximately 1ng/ml as a capture antibody.")
