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Bassoon Antibody (SAP7F407) [Alexa Fluor® 750]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
SAP7F407
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 750

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB120-13249AF350
Alexa Fluor 532
NB120-13249AF532
Alexa Fluor 594
NB120-13249AF594
Biotin
NB120-13249B
DyLight 550
NB120-13249R
DyLight 594
NB120-13249DL594
DyLight 650
NB120-13249C
FITC
NB120-13249F
HRP
NB120-13249H
Janelia Fluor 549
NB120-13249JF549
Janelia Fluor 646
NB120-13249JF646
Unconjugated
NB120-13249
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Bassoon Antibody (SAP7F407) [Alexa Fluor® 750] Summary

Immunogen
Recombinant rat Bassoon.
Specificity
This affinity purified detects an ~400 kDa protein, corresponding to the apparent molecular mass of Bassoon on SDSPAGE immunoblots, in samples from mouse and rat origins. Additional bands between 97 and 400 kDa may also be detected.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BSN
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Bassoon Antibody (SAP7F407) [Alexa Fluor® 750]

  • bassoon (presynaptic cytomatrix protein)

Background

Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle (1-3). Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96% (1). The human BASSOON gene has recently been cloned and localized (3). Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains (1). The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration (1,3). Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors (2). Another presynaptic protein, Piccolo (4) was found to colocalize with Bassoon in cultured hippocampal neurons (1). These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bassoon Antibody
(SAP7F407) [Alexa Flu ...
NB120-13249AF750
Bassoon Antibody (SAP7F407) [Alexa Fluor® 750]
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IP

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Bassoon Antibody (NB120-13249AF750). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for a good antibody to detect Bassoon in mouse brain slices (PFA fixed); can you recommend any one for this?
    • After searching through our product catalog list, we have found the following antibodies which may be of interest: Bassoo. Using the filters on the left column of the site should allow you to filter by species and desired application. Any antibody that is listed to show validation for use in IHC-P and has also been validated in mouse samples will be backed by our 100% Guarantee. Under the terms and conditions of the guarantee, Novus will provide you with a full refund or replacement should the antibody fail to perform, as specified, in samples expressing the protein.

Secondary Antibodies

 

Isotype Controls

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Array NB120-13249AF750

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Bioinformatics

Gene Symbol BSN