![Western Blot: BAK Antibody (AT38E2) [NBP1-74026] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : A431 cell lysateLane 4 : A549 cell lysateLane 5 : Jurkat cell lysateLane 6 : MCF7 cell lysateLane 7 : PC3 cell lysate](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Western-Blot-NBP1-74026-img0009.jpg "Western Blot: BAK Antibody (AT38E2) [NBP1-74026] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : A431 cell lysateLane 4 : A549 cell lysateLane 5 : Jurkat cell lysateLane 6 : MCF7 cell lysateLane 7 : PC3 cell lysate")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clone | 38E2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant human BAK (29-187aa) purified from E.coli |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BAK1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 10% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: BAK Antibody (AT38E2) [NBP1-74026] - Analysis of BAK1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HeLa antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Immunocytochemistry-Immunofluorescence-NBP1-74026-img0006.jpg "Immunocytochemistry/Immunofluorescence: BAK Antibody (AT38E2) [NBP1-74026] - Analysis of BAK1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HeLa antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)")
![Flow Cytometry: BAK Antibody (AT38E2) [NBP1-74026] - Flow Cytometry: BAK Antibody (8B4) [PE] [NBP1-74026PE] - An intracellular stain was performed on Jurkat cells with BAK antibody (8B4) NBP1-74026PE (blue) and a matched isotype control NB600-985PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Flow-Cytometry-NBP1-74026-img0011.jpg "Flow Cytometry: BAK Antibody (AT38E2) [NBP1-74026] - Flow Cytometry: BAK Antibody (8B4) [PE] [NBP1-74026PE] - An intracellular stain was performed on Jurkat cells with BAK antibody (8B4) NBP1-74026PE (blue) and a matched isotype control NB600-985PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE form of this antibody.")
![Flow Cytometry: BAK Antibody (AT38E2) [NBP1-74026] - Analysis of BAK1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Flow-Cytometry-NBP1-74026-img0007.jpg "Flow Cytometry: BAK Antibody (AT38E2) [NBP1-74026] - Analysis of BAK1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).")
![Flow Cytometry: BAK Antibody (AT38E2) [NBP1-74026] - Flow Cytometry: BAK Antibody (8B4) [PE] [NBP1-74026PE] - An intracellular stain was performed on RAW 246.7 cells with BAK antibody (8B4) NBP1-74026PE (blue) and a matched isotype control NB600-985PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Flow-Cytometry-NBP1-74026-img0010.jpg "Flow Cytometry: BAK Antibody (AT38E2) [NBP1-74026] - Flow Cytometry: BAK Antibody (8B4) [PE] [NBP1-74026PE] - An intracellular stain was performed on RAW 246.7 cells with BAK antibody (8B4) NBP1-74026PE (blue) and a matched isotype control NB600-985PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE form of this antibody.")
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