![Flow Cytometry: B7-H7/HHLA2 Antibody (907834) - Azide Free [MAB80842] - An intracellular stain was performed on HeLa cells with B7-H7/HHLA2 [907834] Antibody MAB80842AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/B7-H7-HHLA2-Antibody-907834-Azide-Free-Flow-Cytometry-MAB80842-img0002.jpg "Flow Cytometry: B7-H7/HHLA2 Antibody (907834) - Azide Free [MAB80842] - An intracellular stain was performed on HeLa cells with B7-H7/HHLA2 [907834] Antibody MAB80842AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, CyTOF-ready |
| Clone | 907834 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | >90% by SDS PAGE |
| Immunogen | Partial recombinant human B7-H7 protein (amino acids 1-344) [UniProt Q9UM44] |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HHLA2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for B7-H7/HHLA2 Antibody (MAB80842)Find related products by research area.
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| Gene Symbol | HHLA2 |
![Flow Cytometry: B7-H7/HHLA2 Antibody (907834) - Azide Free [MAB80842] - An intracellular stain was performed on Caco-2 cells with B7-H7/HHLA2 Antibody [907834] MAB80842AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/B7-H7-HHLA2-Antibody-907834-Azide-Free-Flow-Cytometry-MAB80842-img0001.jpg "Flow Cytometry: B7-H7/HHLA2 Antibody (907834) - Azide Free [MAB80842] - An intracellular stain was performed on Caco-2 cells with B7-H7/HHLA2 Antibody [907834] MAB80842AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")