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Autoimmune Regulator/AIRE Antibody (609930) [Alexa Fluor® 405]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clone
609930
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 405

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB6184U
Alexa Fluor 350
IC6184U-100UG
Alexa Fluor 405
IC6184V-100UG
Alexa Fluor 488
IC6184G-100UG
Alexa Fluor 488
FAB6184G
Alexa Fluor 532
FAB6184X
Alexa Fluor 594
IC6184T-100UG
Alexa Fluor 594
FAB6184T
Alexa Fluor 647
IC6184R-100UG
Alexa Fluor 647
FAB6184R
Alexa Fluor 700
IC6184N-100UG
Alexa Fluor 700
FAB6184N
Alexa Fluor 750
FAB6184S
Alexa Fluor 750
IC6184S-100UG
Biotin
FAB6184B
DyLight 350
FAB6184D
DyLight 405
FAB6184E
DyLight 488
FAB6184K
DyLight 550
FAB6184L
DyLight 594
FAB6184M
DyLight 650
FAB6184W
DyLight 680
FAB6184Y
DyLight 755
FAB6184Z
FITC
FAB6184F
HRP
FAB6184H
Janelia Fluor 549
FAB6184I
Janelia Fluor 646
FAB6184J
PE
FAB6184P
PerCP
FAB6184C
Unconjugated
MAB6184
mFluor Violet 450 SE
FAB6184MFV450
mFluor Violet 500 SE
FAB6184MFV500
mFluor Violet 610 SE
FAB6184MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB6184
Carrier Free
MAB6184
Carrier Free
MAB6184-SP
Carrier Free
IC6184N-100UG
Carrier Free
IC6184V-100UG
Carrier Free
IC6184U-100UG
Carrier Free
IC6184T-100UG
Carrier Free
IC6184S-100UG
Carrier Free
IC6184R-100UG
Carrier Free
IC6184G-100UG
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Autoimmune Regulator/AIRE Antibody (609930) [Alexa Fluor® 405] Summary

Immunogen
E. coli-derived recombinant mouse AIRE
Ser476-Ser552 (predicted)
Accession # Q9Z0E3
Specificity
Detects mouse AIRE in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human AIRE is observed.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
AIRE
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Autoimmune Regulator/AIRE Antibody (609930) [Alexa Fluor® 405]

  • AIRE
  • APECED protein
  • APECED
  • APECEDAPSI
  • APS1
  • APSI
  • Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein
  • autoimmune regulator (APECED protein)10APS1AIRE1
  • autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermaldystrophy)
  • Autoimmune Regulator
  • PGA1

Background

AIRE encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAB6184V
Autoimmune Regulator/AIRE Antibody (609930) [Alexa Fluor® 405]
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Product General Protocols

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Video Protocols

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FAQs for Autoimmune Regulator/AIRE Antibody (FAB6184V). (Showing 1 - 1 of 1 FAQ).

  1. I am looking into a positive control for Western blotting. We are trying to detect the AIRE protein and are not sure whether the antibody we have is picking up the protein. Are you able to tell me whether the human thymus whole tissue lysate (NB820-59456) has been used to detect for AIRE expression by Western blotting?
    • I am not currently aware of any references to the use of our human thymus whole tissue lysate being used to detect AIRE by Western blotting. On looking through the Western blot images for all seven of our AIRE antibodies, I see that these have been generated using lysates from RT-4 cells (bladder), U-251 MG cells (brain), 293 cells (human embryonic kidney), human spleen lysate, liver lysate and tonsil lysate. Additionally, you may find the Human Protein Atlas link to AIRE useful. At this site you can find protein expression profiles based on immunohistochemistry for a large number of human tissues, cancers and cell lines: The Human Protein Atlas.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol AIRE