Autoimmune Regulator/AIRE Antibody (609930) [mFluor Violet 450 SE] Summary
| Immunogen |
E. coli-derived recombinant mouse AIRE
Ser476-Ser552 (predicted)
Accession # Q9Z0E3 |
| Specificity |
Detects mouse AIRE in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human AIRE is observed.
|
| Isotype |
IgG2a |
| Clonality |
Monoclonal |
| Host |
Rat |
| Gene |
AIRE |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- CyTOF-ready
- Intracellular Staining by Flow Cytometry
- Western Blot
|
| Application Notes |
Optimal dilution of this antibody should be experimentally determined. |
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
| Buffer |
50mM Sodium Borate |
| Preservative |
0.05% Sodium Azide |
Notes
mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for Autoimmune Regulator/AIRE Antibody (609930) [mFluor Violet 450 SE]
Background
AIRE encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
Publications for Autoimmune Regulator/AIRE Antibody (FAB6184MFV450) (0)
There are no publications for Autoimmune Regulator/AIRE Antibody (FAB6184MFV450).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Autoimmune Regulator/AIRE Antibody (FAB6184MFV450) (0)
There are no reviews for Autoimmune Regulator/AIRE Antibody (FAB6184MFV450).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Autoimmune Regulator/AIRE Antibody (FAB6184MFV450). (Showing 1 - 1 of 1 FAQ).
-
I am looking into a positive control for Western blotting. We are trying to detect the AIRE protein and are not sure whether the antibody we have is picking up the protein. Are you able to tell me whether the human thymus whole tissue lysate (NB820-59456) has been used to detect for AIRE expression by Western blotting?
- I am not currently aware of any references to the use of our human thymus whole tissue lysate being used to detect AIRE by Western blotting. On looking through the Western blot images for all seven of our AIRE antibodies, I see that these have been generated using lysates from RT-4 cells (bladder), U-251 MG cells (brain), 293 cells (human embryonic kidney), human spleen lysate, liver lysate and tonsil lysate. Additionally, you may find the Human Protein Atlas link to AIRE useful. At this site you can find protein expression profiles based on immunohistochemistry for a large number of human tissues, cancers and cell lines: The Human Protein Atlas.
Secondary Antibodies
| |
Isotype Controls
|
Additional Autoimmune Regulator/AIRE Products
Research Areas for Autoimmune Regulator/AIRE Antibody (FAB6184MFV450)
Find related products by research area.
|
Blogs on Autoimmune Regulator/AIRE
