I am looking into a positive control for Western blotting. We are trying to detect the AIRE protein and are not sure whether the antibody we have is picking up the protein. Are you able to tell me whether the human thymus whole tissue lysate (NB820-59456) has been used to detect for AIRE expression by Western blotting?

Name: Autoimmune Regulator/AIRE Antibody (609930) [Janelia Fluor&#174; 585]
Brand: Novus Biologicals
SKU: FAB6184JF585
Price: 519 USD

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Accepted Answer

I am not currently aware of any references to the use of our human thymus whole tissue lysate being used to detect AIRE by Western blotting. On looking through the Western blot images for all seven of our AIRE antibodies, I see that these have been generated using lysates from RT-4 cells (bladder), U-251 MG cells (brain), 293 cells (human embryonic kidney), human spleen lysate, liver lysate and tonsil lysate. Additionally, you may find the Human Protein Atlas link to AIRE useful. At this site you can find protein expression profiles based on immunohistochemistry for a large number of human tissues, cancers and cell lines: The Human Protein Atlas.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	MuSpecies Glossary
Applications	WB, CyTOF-ready, Flow
Clone	609930
Clonality	Monoclonal
Host	Rat
Conjugate	Janelia Fluor 585

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 IC6184U-100UG

Alexa Fluor 405 IC6184V-100UG

Alexa Fluor 488 IC6184G-100UG

Alexa Fluor 532 FAB6184X

Alexa Fluor 594 IC6184T-100UG

Alexa Fluor 647 IC6184R-100UG

Alexa Fluor 700 IC6184N-100UG

Alexa Fluor 750 IC6184S-100UG

Biotin FAB6184B

CoraFluor 1 FAB6184CL1

DyLight 350 FAB6184D

DyLight 405 FAB6184E

DyLight 488 FAB6184K

DyLight 550 FAB6184L

DyLight 594 FAB6184M

DyLight 650 FAB6184W

DyLight 680 FAB6184Y

DyLight 755 FAB6184Z

FITC FAB6184F

HRP FAB6184H

Janelia Fluor 525 FAB6184JF525

Janelia Fluor 549 FAB6184I

Janelia Fluor 635 FAB6184JF635

Janelia Fluor 646 FAB6184J

Janelia Fluor 669 FAB6184JF669

PE FAB6184P

PerCP FAB6184C

Unconjugated MAB6184

mFluor Violet 450 SE FAB6184MFV450

mFluor Violet 500 SE FAB6184MFV500

mFluor Violet 610 SE FAB6184MFV610

Catalog# & Formulation	Size	Price
Standard Formulation MAB6184

Carrier Free MAB6184

Carrier Free MAB6184-SP

Carrier Free IC6184N-100UG

Carrier Free IC6184V-100UG

Carrier Free IC6184U-100UG

Carrier Free IC6184T-100UG

Carrier Free IC6184S-100UG

Carrier Free IC6184R-100UG

Carrier Free IC6184G-100UG

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC6184U-100UG
Alexa Fluor 405	IC6184V-100UG
Alexa Fluor 488	IC6184G-100UG
Alexa Fluor 532	FAB6184X
Alexa Fluor 594	IC6184T-100UG
Alexa Fluor 647	IC6184R-100UG
Alexa Fluor 700	IC6184N-100UG
Alexa Fluor 750	IC6184S-100UG
Biotin	FAB6184B
CoraFluor 1	FAB6184CL1
DyLight 350	FAB6184D
DyLight 405	FAB6184E
DyLight 488	FAB6184K
DyLight 550	FAB6184L
DyLight 594	FAB6184M
DyLight 650	FAB6184W
DyLight 680	FAB6184Y
DyLight 755	FAB6184Z
FITC	FAB6184F
HRP	FAB6184H
Janelia Fluor 525	FAB6184JF525
Janelia Fluor 549	FAB6184I
Janelia Fluor 635	FAB6184JF635
Janelia Fluor 646	FAB6184J
Janelia Fluor 669	FAB6184JF669
PE	FAB6184P
PerCP	FAB6184C
Unconjugated	MAB6184
mFluor Violet 450 SE	FAB6184MFV450
mFluor Violet 500 SE	FAB6184MFV500
mFluor Violet 610 SE	FAB6184MFV610

Autoimmune Regulator/AIRE Antibody (609930) [Janelia Fluor® 585]

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Immunogen	E. coli-derived recombinant mouse AIRE Ser476-Ser552 (predicted) Accession # Q9Z0E3
Specificity	Detects mouse AIRE in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human AIRE is observed.
Isotype	IgG2a
Clonality	Monoclonal
Host	Rat
Gene	AIRE
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	CyTOF-ready Intracellular Staining by Flow Cytometry Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide