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Recombinant Mouse Aspartate Aminotransferase His Protein

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SDS-Page: Recombinant Mouse Aspartate Aminotransferase Protein [NBP2-52088]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE, Enzyme Activity
Concentration
0.5 mg/ml

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Recombinant Mouse Aspartate Aminotransferase His Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-413 of Mouse Aspartate Aminotransferase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAPPSVF AQVPQAPPVL VFKLTADFRD DPDPRKVNLG VGAYRTDESQ PWVLPVVRKV EQKIANDNSL NHEYLPILGL AEFRSCASRL VLGDNSPAIR ENRVGGVQSL GGTGALRIGA DFLGRWYNGT DNKNTPIYVS SPTWENHNAV FSAAGFKDIR PYCYWDAEKR GLDLQGFLND LENAPEFSIF VLHACAHNPT GTDPTPEQWK QIAAVMQRRF LFPFFDSAYQ GFASGDLEKD AWAIRYFVSE GFELFCAQSF SKNFGLYNER VGNLTVVGKE SDSVLRVLSQ MEKIVRITWS NPPAQGARIV AATLSDPELF KEWKGNVKTM ADRILTMRSE LRARLEALKT PGTWSHITEQ IGMFSFTGLN PKQVEYLVNE KHIYLLPSGR INMCGLTTKN LDYVATSIHE AVTKIQ

Details of Functionality
Specific activity is > 80 units/mg, and is defined as the amount of enzyme that converts 1.0 micromole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GOT1
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Enzyme Activity
  • SDS-Page
Theoretical MW
48.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% glycerol, 1 mM DTT
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Mouse Aspartate Aminotransferase His Protein

  • aspartate aminotransferase, cytoplasmic
  • ASTQTL1
  • CASPAT
  • CCAT
  • Cysteine aminotransferase, cytoplasmic
  • Cysteine transaminase, cytoplasmic
  • EC 2.6.1.1
  • EC 2.6.1.3
  • GIG18
  • Glutamate oxaloacetate transaminase 1
  • glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
  • GOT1
  • growth-inhibiting protein 18
  • Transaminase A

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol GOT1