Reactivity | MuSpecies Glossary |
Applications | PAGE, Enzyme Activity |
Concentration | 0.5 mg/ml |
Description | A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-413 of Mouse Aspartate Aminotransferase Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAPPSVF AQVPQAPPVL VFKLTADFRD DPDPRKVNLG VGAYRTDESQ PWVLPVVRKV EQKIANDNSL NHEYLPILGL AEFRSCASRL VLGDNSPAIR ENRVGGVQSL GGTGALRIGA DFLGRWYNGT DNKNTPIYVS SPTWENHNAV FSAAGFKDIR PYCYWDAEKR GLDLQGFLND LENAPEFSIF VLHACAHNPT GTDPTPEQWK QIAAVMQRRF LFPFFDSAYQ GFASGDLEKD AWAIRYFVSE GFELFCAQSF SKNFGLYNER VGNLTVVGKE SDSVLRVLSQ MEKIVRITWS NPPAQGARIV AATLSDPELF KEWKGNVKTM ADRILTMRSE LRARLEALKT PGTWSHITEQ IGMFSFTGLN PKQVEYLVNE KHIYLLPSGR INMCGLTTKN LDYVATSIHE AVTKIQ |
Details of Functionality | Specific activity is > 80 units/mg, and is defined as the amount of enzyme that converts 1.0 micromole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C. |
Source | E. coli |
Protein/Peptide Type | Recombinant Protein |
Gene | GOT1 |
Purity | >95%, by SDS-PAGE |
Dilutions |
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Theoretical MW | 48.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | PBS (pH 7.4), 10% glycerol, 1 mM DTT |
Preservative | No Preservative |
Concentration | 0.5 mg/ml |
Purity | >95%, by SDS-PAGE |
Research Areas for Aspartate Aminotransferase Recombinant Protein (NBP2-52088)Find related products by research area.
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Gene Symbol | GOT1 |