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![Western Blot: Aspartate Aminotransferase Antibody [NBP1-54778] - Titration: 1.0ug/ml, Positive Control: NCI-H226 cell lysate.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Western-Blot-NBP1-54778-img0006.jpg "Western Blot: Aspartate Aminotransferase Antibody [NBP1-54778] - Titration: 1.0ug/ml, Positive Control: NCI-H226 cell lysate.")
![Western Blot: Aspartate Aminotransferase Antibody [NBP1-54778] - Human Fetal Liver.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Western-Blot-NBP1-54778-img0004.jpg)
![Western Blot: Aspartate Aminotransferase Antibody [NBP1-54778] - Human NCI-H226.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Western-Blot-NBP1-54778-img0005.jpg)
![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-57518] - Staining in human heart muscle and lymph node tissues using anti-GOT1 antibody. Corresponding GOT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NBP2-57518-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-57518] - Staining in human heart muscle and lymph node tissues using anti-GOT1 antibody. Corresponding GOT1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Aspartate Aminotransferase Antibody [NBP2-57518] - Analysis using Anti-GOT1 antibody NBP2-57518 (A) shows similar pattern to independent antibody NBP2-55813 (B).](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Western-Blot-NBP2-57518-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Aspartate Aminotransferase Antibody [NBP2-57518] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57518-img0001.jpg)
![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-57518] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NBP2-57518-img0004.jpg)
![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-57518] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NBP2-57518-img0005.jpg)
![Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-101220241252813.jpg "Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Human colon using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-101220241233823.jpg)
![Western Blot: Aspartate Aminotransferase Antibody [NBP3-38190] - Western blot analysis of various lysates using Aspartate Aminotransferase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-1012202411592816.jpg)
![Immunohistochemistry: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunohistochemistry analysis of paraffin-embedded Rat heart using Aspartate Aminotransferase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-10122024120650.jpg)
![Immunocytochemistry/ Immunofluorescence: Aspartate Aminotransferase Antibody [NBP3-38190] - Immunofluorescence analysis of U2OS cells using Aspartate Aminotransferase Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38190_rabbit-aspartate-aminotransferase-pab-101220241243928.jpg)
![Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - The recombinat proteins (100ng) were resolved by SDS PAGE, transferred to PVDF membrane and probed with antihuman GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant human GOT1 Lane 2 : Recombinant human GOT2](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Western-Blot-NBP2-59483-img0009.jpg "Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - The recombinat proteins (100ng) were resolved by SDS PAGE, transferred to PVDF membrane and probed with antihuman GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant human GOT1 Lane 2 : Recombinant human GOT2")
![Immunocytochemistry/Immunofluorescence: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Analysis of GOT1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of GOT1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Immunocytochemistry-Immunofluorescence-NBP2-59483-img0002.jpg)
![Flow Cytometry: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Flow cytometry analysis of GOT1 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Flow-Cytometry-NBP2-59483-img0006.jpg)
![Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Lane 1: 293T cell lysates, Lane 2: GOT1 Transfected 293T cell lysates](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Western-Blot-NBP2-59483-img0001.jpg)
![Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : A427 cell lysate](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Western-Blot-NBP2-59483-img0008.jpg)
![Flow Cytometry: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Flow cytometry analysis of GOT1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Flow-Cytometry-NBP2-59483-img0003.jpg)
![Western Blot: Aspartate Aminotransferase Antibody [NB100-93403] - Staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Western-Blot-NB100-93403-img0003.jpg "Western Blot: Aspartate Aminotransferase Antibody [NB100-93403] - Staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NB100-93403] - Staining of human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NB100-93403-img0002.jpg)
![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NB100-93403] - Staining of human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NB100-93403-img0001.jpg)
![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-55813] - Staining in human heart muscle and lymph node tissues using anti-GOT1 antibody. Corresponding GOT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NBP2-55813-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-55813] - Staining in human heart muscle and lymph node tissues using anti-GOT1 antibody. Corresponding GOT1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Aspartate Aminotransferase Antibody [NBP2-55813] - Analysis using Anti-GOT1 antibody NBP2-55813 (A) shows similar pattern to independent antibody NBP2-57518 (B).](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Western-Blot-NBP2-55813-img0006.jpg)
![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-55813] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NBP2-55813-img0003.jpg)
![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-55813] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NBP2-55813-img0004.jpg)
![Western Blot: Aspartate Aminotransferase Antibody (5J1B4) [NBP3-15347] - Western blot analysis of extracts of various cell lines, using Aspartate Aminotransferase Rabbit mAb (NBP3-15347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-5J1B4-Western-Blot-NBP3-15347-img0002.jpg "Western Blot: Aspartate Aminotransferase Antibody (5J1B4) [NBP3-15347] - Western blot analysis of extracts of various cell lines, using Aspartate Aminotransferase Rabbit mAb (NBP3-15347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Aspartate Aminotransferase Antibody (5J1B4) [NBP3-15347] - Immunofluorescence analysis of HeLa cells using Aspartate Aminotransferase Rabbit mAb (NBP3-15347) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-5J1B4-Immunocytochemistry-Immunofluorescence-NBP3-15347-img0001.jpg)
![Western Blot: Aspartate Aminotransferase Antibody (5J1B4) [NBP3-15347] - Western blot analysis of extracts of various cell lines, using Aspartate Aminotransferase Rabbit mAb (NBP3-15347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-5J1B4-Western-Blot-NBP3-15347-img0003.jpg)
![Aspartate Transaminase Assay Kit (Colorimetric) [KA1625] - Purified AST enzyme (assayed at 37 degrees C).](http://images.novusbio.com/fullsize/Aspartate-Transaminase-Assay-Kit-Colorimetric-KA1625-img0001.jpg "Aspartate Transaminase Assay Kit (Colorimetric) [KA1625] - Purified AST enzyme (assayed at 37 degrees C).")
![ELISA: Mouse Aspartate Aminotransferase ELISA Kit (Colorimetric) [NBP2-69877] - Samples were spiked with high concentrations of Mouse Aspartate Aminotransferase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69877_mouse-aspartate-aminotransferase-elisa-kit-colorimetric-132202416201912.jpg "ELISA: Mouse Aspartate Aminotransferase ELISA Kit (Colorimetric) [NBP2-69877] - Samples were spiked with high concentrations of Mouse Aspartate Aminotransferase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Aspartate Aminotransferase ELISA Kit (Colorimetric) [NBP2-69877] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69877_mouse-aspartate-aminotransferase-elisa-kit-colorimetric-43202494150.jpg)
![Western Blot: Aspartate Aminotransferase Antibody (SR1790) [NBP3-21842] - Western blot analysis using NBP3-21842 in MCF7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21842_rabbit-aspartate-aminotransferase-mab-sr1790-57202316582111.jpg "Western Blot: Aspartate Aminotransferase Antibody (SR1790) [NBP3-21842] - Western blot analysis using NBP3-21842 in MCF7 cell lysate.")
![Immunohistochemistry: Aspartate Aminotransferase Antibody (2F1) [NBP3-42977] - Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using (Aspartate Aminotransferase Antibody) at dilution of 1:200](http://images.novusbio.com/fullsize/nbp3-42977_mouse-aspartate-aminotransferase-mab-2f1-101220241723660.jpg "Immunohistochemistry: Aspartate Aminotransferase Antibody (2F1) [NBP3-42977] - Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using (Aspartate Aminotransferase Antibody) at dilution of 1:200")
![Western Blot: Aspartate Aminotransferase Antibody (2F1) [NBP3-42977] - human brain tissue were subjected to SDS PAGE followed by western blot with (Aspartate Aminotransferase Antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-42977_mouse-aspartate-aminotransferase-mab-2f1-101220241734153.jpg)
![Western Blot: Aspartate Aminotransferase Antibody (A2D6-R) [NBP3-32050] - Western blot analysis of Aspartate Aminotransferase on different lysates with Mouse anti-Aspartate Aminotransferase antibody (NBP3-32050) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: HL-60 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Mouse liver tissue lysate (40 ug/Lane) Lane 7: Mouse heart tissue lysate (40 ug/Lane) Lane 8: Rat liver tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 46 kDa Observed band size: 38 kDa Exposure time: 1 minute 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32050) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32050_mouse-aspartate-aminotransferase-mab-a2d6-r-278202416331228.jpg "Western Blot: Aspartate Aminotransferase Antibody (A2D6-R) [NBP3-32050] - Western blot analysis of Aspartate Aminotransferase on different lysates with Mouse anti-Aspartate Aminotransferase antibody (NBP3-32050) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: HL-60 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Mouse liver tissue lysate (40 ug/Lane) Lane 7: Mouse heart tissue lysate (40 ug/Lane) Lane 8: Rat liver tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 46 kDa Observed band size: 38 kDa Exposure time: 1 minute 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32050) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")