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Aspartate Aminotransferase Activity Assay Kit (Fluorometric)

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Aspartate Aminotransferase Activity Assay Kit (Fluorometric) [NBP3-24471] - Standard Reference Curve
Aspartate Aminotransferase Activity Assay Kit (Fluorometric) [NBP3-24471] - Assay Example

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications Func
Suitable Sample Type
Serum, plasma, animal tissue and cell samples
Standard Curve Range
0.03 - 1.5 U/L
Sensitivity
0.03 U/L

Order Details

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Aspartate Aminotransferase Activity Assay Kit (Fluorometric) Summary

Description
Detection instrument: Fluorescence microplate reader (Ex/Em = 535 nm/587 nm)
Assay time: 80 min
Standard Curve Range
0.03 - 1.5 U/L
Sensitivity
0.03 U/L
Inter-Assay
%CV < 7.3
Intra-Assay
%CV < 2.2
Spike Recovery
Average recovery rate: 100%
Kit Type
Activity Assay Kit (Fluorometric)
Gene
GOT1

Applications/Dilutions

Dilutions
  • Functional

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Black Microplate
  2. Plate Sealer
  3. Reagent 1: Buffer Solution
  4. Reagent 2: Substrate
  5. Reagent 3: Enzyme Reagent
  6. Reagent 4: Aqueous Alkali
  7. Reagent 5: 100 mmol/L Pyruvate Standard

Alternate Names for Aspartate Aminotransferase Activity Assay Kit (Fluorometric)

  • aspartate aminotransferase, cytoplasmic
  • ASTQTL1
  • CASPAT
  • CCAT
  • Cysteine aminotransferase, cytoplasmic
  • Cysteine transaminase, cytoplasmic
  • EC 2.6.1.1
  • EC 2.6.1.3
  • GIG18
  • Glutamate oxaloacetate transaminase 1
  • glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
  • GOT1
  • growth-inhibiting protein 18
  • Transaminase A

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol GOT1