Aspartate Aminotransferase Activity Assay Kit (Colorimetric) Summary
Description |
Detection instrument: Microplate reader (500 - 520 nm) Assay time: 80 min |
Standard Curve Range |
1.1 - 72.3 IU/L |
Sensitivity |
1.1 IU/L |
Inter-Assay |
%CV < 6.8 |
Intra-Assay |
%CV < 5.3 |
Kit Type |
Activity Assay Kit (Colorimetric) |
Gene |
GOT1 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Microplate
- Plate Sealer
- Reagent 1: Buffer Solution
- Reagent 2: 2 mmol/L Sodium Pyruvate
- Reagent 3: Substrate Solution
- Reagent 4: Chromogenic Agent
- Reagent 5: Alkali Reagent
|
Alternate Names for Aspartate Aminotransferase Activity Assay Kit (Colorimetric)
Background
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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