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Mouse Aspartate Aminotransferase ELISA Kit (Colorimetric)

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ELISA: Mouse Aspartate Aminotransferase ELISA Kit (Colorimetric) [NBP2-69877] - Samples were spiked with high concentrations of Mouse Aspartate Aminotransferase and diluted with Reference Standard & Sample Diluent to ...read more
ELISA: Mouse Aspartate Aminotransferase ELISA Kit (Colorimetric) [NBP2-69877] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

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Mouse Aspartate Aminotransferase ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse AST in samples. No significant cross-reactivity or interference between Mouse AST and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.42%
Intra-Assay
CV% < 4.84%
Spike Recovery
88-108%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GOT1

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publications using NBP2-69877.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse Aspartate Aminotransferase ELISA Kit (Colorimetric)

  • aspartate aminotransferase, cytoplasmic
  • ASTQTL1
  • CASPAT
  • CCAT
  • Cysteine aminotransferase, cytoplasmic
  • Cysteine transaminase, cytoplasmic
  • EC 2.6.1.1
  • EC 2.6.1.3
  • GIG18
  • Glutamate oxaloacetate transaminase 1
  • glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
  • GOT1
  • growth-inhibiting protein 18
  • Transaminase A

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Aspartate Aminotransferase ELISA Kit (NBP2-69877)(3)

We have publications tested in 1 application: ELISA.


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Showing Publications 1 - 3 of 3.
Publications using NBP2-69877 Applications Species
Wang Z, Jiang X, Zhang L, Chen H Protective effects of Althaea officinalis L. extract against N ?diethylnitrosamine?induced hepatocellular carcinoma in male Wistar rats through antioxidative, anti?inflammatory, mitochondrial apoptosis and PI3K /Akt/ mTOR signaling pathways Food Science & Nutrition 2023-05-26 (ELISA) ELISA
Zhenqian Wang, Xiao Jiang, Long Zhang, Han Chen Protective effects of Althaea officinalis L. extract against N ‐diethylnitrosamine‐induced hepatocellular carcinoma in male Wistar rats through antioxidative, anti‐inflammatory, mitochondrial apoptosis and PI3K /Akt/ mTOR signaling pathways Food Science & Nutrition 2023-05-26 [PMID: 37576045]
Li J, Diamante G, Ahn I et al. Determination of the nanoparticle- and cell-specific toxicological mechanisms in 3D liver spheroids using scRNAseq analysis Nano Today 2022-12-01 [PMID: 36911538]

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Bioinformatics

Gene Symbol GOT1