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Human Aspartate Aminotransferase ELISA Kit (Colorimetric)

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ELISA: Human Aspartate Aminotransferase ELISA Kit (Colorimetric) [NBP2-69876] - Samples were spiked with high concentrations of Human Aspartate Aminotransferase and diluted with Reference Standard & Sample Diluent to ...read more
ELISA: Human Aspartate Aminotransferase ELISA Kit (Colorimetric) [NBP2-69876] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

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Human Aspartate Aminotransferase ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human AST in samples. No significant cross-reactivity or interference between Human AST and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.58%
Intra-Assay
CV% < 5.55%
Spike Recovery
89-103%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GOT1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Aspartate Aminotransferase ELISA Kit (Colorimetric)

  • aspartate aminotransferase, cytoplasmic
  • ASTQTL1
  • CASPAT
  • CCAT
  • Cysteine aminotransferase, cytoplasmic
  • Cysteine transaminase, cytoplasmic
  • EC 2.6.1.1
  • EC 2.6.1.3
  • GIG18
  • Glutamate oxaloacetate transaminase 1
  • glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
  • GOT1
  • growth-inhibiting protein 18
  • Transaminase A

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol GOT1