![Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153] - Staining of Human Kidney (A) Mouse Liver (B) with antibody at 0.01 ug/mL and Rat Kidney (C) lysate with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-00153-img0005.jpg "Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153] - Staining of Human Kidney (A) Mouse Liver (B) with antibody at 0.01 ug/mL and Rat Kidney (C) lysate with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
| Reactivity | Hu, Rt, Bv, Mu, CaSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-ENPKNQAPPGLYTKTQD corresponding to internal region according to NP_446464.1. |
| Specificity | The variants represent identical protein (NP_000041.2 and NP_446464.1). |
| Predicted Species | Mouse (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | ASS1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IP successful usage from BAEC cells is reported in scientific literature (PMID: 18948083). WB: Approx. 45 kDa band observed in human kidney lysates (calculated MW of 46.5 kDa band according to NP_000041.2 and NP_446464.1). IHC-P: Human kidney shows textured cytoplasm staining in PCT. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Argininosuccinate Synthase Antibody (NBP1-00153)Find related products by research area.
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| Gene Symbol | ASS1 |
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| Uniprot |
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![Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody [NBP1-00153] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00153-img0006.jpg "Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody [NBP1-00153] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153] - (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-00153-img0003.jpg "Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153] - (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Flow Cytometry: Argininosuccinate Synthase Antibody [NBP1-00153] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Flow-Cytometry-NBP1-00153-img0007.jpg "Flow Cytometry: Argininosuccinate Synthase Antibody [NBP1-00153] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153] - Staining of A431 (A) and (1ug/ml) NIH3T3(B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-00153-img0004.jpg "Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153] - Staining of A431 (A) and (1ug/ml) NIH3T3(B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153] - (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-00153-img0002.jpg "Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153] - (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
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