Argininosuccinate Synthase Antibodies
We offer Argininosuccinate Synthase Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot. Each Argininosuccinate Synthase Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Argininosuccinate Synthase Antibodies can be used in a variety of model species: Bovine, Human, Monkey, Mouse, Rat. Use the list below to choose the Argininosuccinate Synthase Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Argininosuccinate Synthase polyclonal antibodies and browse our Argininosuccinate Synthase monoclonal antibody catalog.
Alternate Names for Argininosuccinate Synthase Antibodies
anti-Argininosuccinate Synthase antibody, anti-ASS1 antibody, anti-argininosuccinate synthase 1 antibody, anti-argininosuccinate synthetase 1 antibody, anti-argininosuccinate synthetase antibody, anti-ASSCTLN1 antibody, anti-Citrulline--aspartate ligase antibody, anti-citrulline-aspartate ligase antibody, anti-EC 6.3.4.5 antibody
14 Results for "argininosuccinate-synthase" in Primary Antibodies
![Western Blot: Argininosuccinate Synthase Antibody [NBP1-88868] - ASS1 was successfully silenced in H4-II-E-C3 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-88868-img0013.jpg "Western Blot: Argininosuccinate Synthase Antibody [NBP1-88868] - ASS1 was successfully silenced in H4-II-E-C3 cells. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88868-img0007.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human skin shows moderate cytoplasmic positivity in a subset of squamous epithelial cells.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0023.jpg)
![Western Blot: Argininosuccinate Synthase Antibody [NBP1-88868] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-88868-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Argininosuccinate Synthase Antibody [NBP1-88868] - Analysis in human cell lines MCF-7 and U-251MG using anti-ASS1 antibody. Corresponding ASS1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-88868-img0012.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0008.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining in human kidney and lymph node tissues using anti-ASS1 antibody. Corresponding ASS1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human kidney, liver, lymph node and urinary bladder using Anti-ASS1 antibody NBP1-88868 (A) shows similar protein distribution across tissues to independent antibody NBP1-88867 (B).](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0014.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human urinary bladder.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0015.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human kidney using Anti-ASS1 antibody NBP1-88868.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0017.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human lymph node using Anti-ASS1 antibody NBP1-88868.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0018.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Analysis in human kidney and lymph node tissues using NBP1-88868 antibody. Corresponding ASS1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0019.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human kidney, liver, lymph node and skin using Anti-ASS1 antibody NBP1-88868 (A) shows similar protein distribution across tissues to independent antibody NBP1-88867 (B).](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0020.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0021.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human lymph node shows no cytoplasmic positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0022.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88868] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88868-img0024.jpg)
![Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153] - Staining of Human Kidney (A) Mouse Liver (B) with antibody at 0.01 ug/mL and Rat Kidney (C) lysate with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-00153-img0005.jpg "Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153] - Staining of Human Kidney (A) Mouse Liver (B) with antibody at 0.01 ug/mL and Rat Kidney (C) lysate with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody [NBP1-00153] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00153-img0006.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153] - (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-00153-img0003.jpg)
![Flow Cytometry: Argininosuccinate Synthase Antibody [NBP1-00153] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Flow-Cytometry-NBP1-00153-img0007.jpg)
![Western Blot: Argininosuccinate Synthase Antibody [NBP1-00153] - Staining of A431 (A) and (1ug/ml) NIH3T3(B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-00153-img0004.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-00153] - (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-00153-img0002.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining in human kidney and lymph node tissues using anti-ASS1 antibody. Corresponding ASS1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88867-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining in human kidney and lymph node tissues using anti-ASS1 antibody. Corresponding ASS1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human kidney, liver, lymph node and urinary bladder using Anti-ASS1 antibody NBP1-88867 (A) shows similar protein distribution across tissues to independent antibody NBP1-88868 (B).](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88867-img0013.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Argininosuccinate Synthase Antibody [NBP1-88867] - Analysis in human cell lines MCF-7 and U-251MG using anti-ASS1 antibody. Corresponding ASS1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-88867-img0011.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human skin shows strong cytoplasmic positivity in a subset of squamous epithelial cells.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88867-img0023.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human lymph node using Anti-ASS1 antibody NBP1-88867.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88867-img0017.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human kidney shows strong cytoplasmic positivity in cells in proximal tubules.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88867-img0019.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88867-img0021.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human lymph node shows no cytoplasmic positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88867-img0022.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-88867-img0024.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (2B10) [NBP2-37520] - Analysis using ASS1 mouse mAb against A431 (1), RAJI (2), L1210 (3), MOLT4 (4), Jurkat (5), A549 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2B10-Western-Blot-NBP2-37520-img0003.jpg "Western Blot: Argininosuccinate Synthase Antibody (2B10) [NBP2-37520] - Analysis using ASS1 mouse mAb against A431 (1), RAJI (2), L1210 (3), MOLT4 (4), Jurkat (5), A549 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody (2B10) [NBP2-37520] - Analysis of Hela cells using ASS1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2B10-Immunocytochemistry-Immunofluorescence-NBP2-37520-img0004.jpg)
![Flow Cytometry: Argininosuccinate Synthase Antibody (2B10) [NBP2-37520] - Analysis of Jurkat cells using ASS1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2B10-Flow-Cytometry-NBP2-37520-img0006.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (2B10) [NBP2-37520] - Analysis using ASS1 mAb against human ASS1 (AA: 40-236) recombinant protein. (Expected MW is 47 kDa)](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2B10-Western-Blot-NBP2-37520-img0002.jpg)
![ELISA: Argininosuccinate Synthase Antibody (2B10) [NBP2-37520] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2B10-ELISA-NBP2-37520-img0001.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (2C10) [NBP2-37518] - Western blot analysis using ASS1 mouse mAb against A431 (1), RAJI (2), MOLT4 (3), Jurkat (4), A549 (5), NIH/3T3 (6), PC-12 (7) and Cos7 (8) cell lysate.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2C10-Western-Blot-NBP2-37518-img0003.jpg "Western Blot: Argininosuccinate Synthase Antibody (2C10) [NBP2-37518] - Western blot analysis using ASS1 mouse mAb against A431 (1), RAJI (2), MOLT4 (3), Jurkat (4), A549 (5), NIH/3T3 (6), PC-12 (7) and Cos7 (8) cell lysate.")
![Immunohistochemistry: Argininosuccinate Synthase Antibody (2C10) [NBP2-37518] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using ASS1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2C10-Immunohistochemistry-NBP2-37518-img0005.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (2C10) [NBP2-37518] - Western blot analysis using ASS1 mAb against human ASS1 (AA: 40-236) recombinant protein. (Expected MW is 47 kDa)](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2C10-Western-Blot-NBP2-37518-img0002.jpg)
![Immunohistochemistry: Argininosuccinate Synthase Antibody (2C10) [NBP2-37518] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ASS1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2C10-Immunohistochemistry-NBP2-37518-img0004.jpg)
![ELISA: Argininosuccinate Synthase Antibody (2C10) [NBP2-37518] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-2C10-ELISA-NBP2-37518-img0001.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (S02-7A8) [NBP3-19945] - Western blot detection of Argininosuccinate Synthase in Hela cell lysates using NBP3-19945 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-S02-7A8-Western-Blot-NBP3-19945-img0003.jpg "Western Blot: Argininosuccinate Synthase Antibody (S02-7A8) [NBP3-19945] - Western blot detection of Argininosuccinate Synthase in Hela cell lysates using NBP3-19945 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.")
![Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody (S02-7A8) [NBP3-19945] - Immunofluorescence of Argininosuccinate Synthase (green) in Hela cells using NBP3-19945 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-S02-7A8-Immunocytochemistry-Immunofluorescence-NBP3-19945-img0001.jpg)
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody (S02-7A8) [NBP3-19945] - Immunohistochemistry of Argininosuccinate Synthase in paraffin-embedded Human breast cancer tissue using NBP3-19945 at dilution 1/1](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-S02-7A8-Immunohistochemistry-Paraffin-NBP3-19945-img0002.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (11F1-RA) [NBP3-32051] - Western blot analysis of Argininosuccinate Synthase on different lysates with Mouse anti-Argininosuccinate Synthase antibody (NBP3-32051) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (10 ug/Lane) Lane 2: THP-1 cell lysate (10 ug/Lane) Lane 3: SiHa cell lysate (10 ug/Lane) Lane 4: Rat kidney tissue lysate (20 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32051) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32051_mouse-argininosuccinate-synthase-mab-11f1-ra-278202416331278.jpg "Western Blot: Argininosuccinate Synthase Antibody (11F1-RA) [NBP3-32051] - Western blot analysis of Argininosuccinate Synthase on different lysates with Mouse anti-Argininosuccinate Synthase antibody (NBP3-32051) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (10 ug/Lane) Lane 2: THP-1 cell lysate (10 ug/Lane) Lane 3: SiHa cell lysate (10 ug/Lane) Lane 4: Rat kidney tissue lysate (20 ug/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32051) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Argininosuccinate Synthase Antibody (11F1-RA) [NBP3-32051] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-Argininosuccinate Synthase antibody (NBP3-32051) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32051) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32051_mouse-argininosuccinate-synthase-mab-11f1-ra-2982024142090.jpg)
![Immunocytochemistry/ Immunofluorescence: Argininosuccinate Synthase Antibody (11F1-RA) [NBP3-32051] - Immunocytochemistry analysis of MCF-7 cells labeling Argininosuccinate Synthase with Mouse anti-Argininosuccinate Synthase antibody (NBP3-32051) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Argininosuccinate Synthase antibody (NBP3-32051) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32051_mouse-argininosuccinate-synthase-mab-11f1-ra-2920241033404.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (4W1M8) [NBP3-16749] - Western blot analysis of extracts of various cell lines, using Argininosuccinate Synthase Rabbit mAb (NBP3-16749) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-4W1M8-Western-Blot-NBP3-16749-img0002.jpg "Western Blot: Argininosuccinate Synthase Antibody (4W1M8) [NBP3-16749] - Western blot analysis of extracts of various cell lines, using Argininosuccinate Synthase Rabbit mAb (NBP3-16749) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody (4W1M8) [NBP3-16749] - Immunofluorescence analysis of NIH-3T3 cells using Argininosuccinate Synthase Rabbit mAb (NBP3-16749) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-4W1M8-Immunocytochemistry-Immunofluorescence-NBP3-16749-img0001.jpg)
![Immunohistochemistry: Argininosuccinate Synthase Antibody (18G11) [NBP3-26499] - Image of Argininosuccinate Synthase Antibody (18G11) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.56% DAB.](http://images.novusbio.com/fullsize/nbp3-26499_rabbit-argininosuccinate-synthase-mab-18g11-262202415475626.jpg "Immunohistochemistry: Argininosuccinate Synthase Antibody (18G11) [NBP3-26499] - Image of Argininosuccinate Synthase Antibody (18G11) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.56% DAB.")
![Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody (18G11) [NBP3-26499] - Staining of Hela with Argininosuccinate Synthase Antibody (18G11) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 516-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26499_rabbit-argininosuccinate-synthase-mab-18g11-28220241247517.jpg)
![Flow Cytometry: Argininosuccinate Synthase Antibody (18G11) [NBP3-26499] - Overlay Peak curve showing Jurkat cells stained with Argininosuccinate Synthase Antibody (18G11) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26499_rabbit-argininosuccinate-synthase-mab-18g11-282202414312780.jpg)
![Western Blot: Argininosuccinate Synthase Antibody [NBP1-31522] - A. 30 ug A431 whole cell lysate/extract 10 % SDS-PAGE Argininosuccinate Synthase antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Western-Blot-NBP1-31522-img0004.jpg "Western Blot: Argininosuccinate Synthase Antibody [NBP1-31522] - A. 30 ug A431 whole cell lysate/extract 10 % SDS-PAGE Argininosuccinate Synthase antibody dilution: 1:1000")
![Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-31522] - Cal27 xenograft, using ASS1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-Immunohistochemistry-Paraffin-NBP1-31522-img0002.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (1G11) [NBP2-59443] - Analysis in MCF-7 cell lysates (40ug), resolved by SDS-PAGE.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-1G11-Western-Blot-NBP2-59443-img0001.jpg "Western Blot: Argininosuccinate Synthase Antibody (1G11) [NBP2-59443] - Analysis in MCF-7 cell lysates (40ug), resolved by SDS-PAGE.")
![Immunocytochemistry/Immunofluorescence: Argininosuccinate Synthase Antibody (1G11) [NBP2-59443] - Analysis of ASS1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ASS1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-1G11-Immunocytochemistry-Immunofluorescence-NBP2-59443-img0002.jpg)
![Western Blot: Argininosuccinate Synthase Antibody (1B10) [NBP2-46551] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Argininosuccinate Synthase.](http://images.novusbio.com/fullsize/Argininosuccinate-Synthase-Antibody-1B10-Western-Blot-NBP2-46551-img0001.jpg "Western Blot: Argininosuccinate Synthase Antibody (1B10) [NBP2-46551] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Argininosuccinate Synthase.")