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Apolipoprotein B100/ApoB100 Antibody [Unconjugated]

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Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Apolipoprotein B100/ApoB100 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3260) followed by HRP-conjugated ...read more
Simple Western shows lysates of human plasma, loaded at 1:50. A specific band was detected for Apolipoprotein B100/ApoB100 at approximately 307 kDa (as indicated) using 5 µg/mL of Goat Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Apolipoprotein B100/ApoB100 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human ApoB100
Ile3374-Val3600
Accession # AAP72970
Specificity
Detects human ApoB100 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
APOB
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 5 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3260 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Apolipoprotein B100/ApoB100 Antibody [Unconjugated]

  • apoB-100;apoB-48;Apolipoprotein B-100;FCHL2;FLDB;LDLCQ4
  • Apolipoprotein B100

Background

ApoB100 is a major serum Apolipoprotein synthesized by the liver. It is an important constituent of LDL, VLDL and IDL particles. It interacts with the LDL receptor and is involved in the delivery of cholesterol to tissues. Within the peptide sequence of the immunogen, human and mouse ApoB100 share 74% amino acid sequence identity. The sequence within the immunogen is absent in ApoB48, an alternative splice isoform that is exclusively synthesized by the gut. ApoB48 contains the amino terminal 47% of ApoB100 and lacks the LDL receptor region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein B100/ApoB100 Antibody (AF3260)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Functional Assay, Western Blot.


Filter By Application
Functional Assay
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF3260 Applications Species
Hyun Ji Kim, Matthew J. Rames, Florian Goncalves, C. Ward Kirschbaum, Breeshey Roskams-Hieter, Elias Spiliotopoulos et al. Selective enrichment of plasma cell-free messenger RNA in cancer-associated extracellular vesicles Communications Biology 2023-08-29 [PMID: 37644220]
Patrick Borchers, Patrick Winnersbach, Sandra Kraemer, Christian Beckers, Eva Miriam Buhl, Steffen Leonhardt et al. Comparison of the Hemocompatibility of an Axial and a Centrifugal Left Ventricular Assist Device in an In Vitro Test Circuit Journal of Clinical Medicine 2022-06-15 [PMID: 35743501]
Xiaogang Zhang, Ellen G. F. Borg, A. Manuel Liaci, Harmjan R. Vos, Willem Stoorvogel A novel three step protocol to isolate extracellular vesicles from plasma or cell culture medium with both high yield and purity Journal of Extracellular Vesicles 2020-09-01 [PMID: 32944179]
Tom A.P. Driedonks, Sanne Mol, Sanne de Bruin, Anna‐Linda Peters, Xiaogang Zhang, Marthe F.S. Lindenbergh et al. Y‐RNA subtype ratios in plasma extracellular vesicles are cell type‐ specific and are candidate biomarkers for inflammatory diseases Journal of Extracellular Vesicles 2020-09-01 [PMID: 32944168]
K Chemello, S Beeské, TT Trang Tran, V Blanchard, EF Villard, B Poirier, JC Le Bail, G Dargazanli, S Ho-Van-Gui, D Boulay, O Bergis, MP Pruniaux, M Croyal, P Janiak, E Guillot, G Lambert Lipoprotein(a) Cellular Uptake Ex�Vivo and Hepatic Capture In�Vivo Is Insensitive to PCSK9 Inhibition With Alirocumab JACC Basic Transl Sci, 2020-05-06;5(6):549-557. 2020-05-06 [PMID: 32613143] (Western Blot, Human) Western Blot Human
Goren Saenz‐Pipaon, Patxi San Martín, Núria Planell, Alberto Maillo, Susana Ravassa, Amaia Vilas‐Zornoza et al. Functional and transcriptomic analysis of extracellular vesicles identifies calprotectin as a new prognostic marker in peripheral arterial disease (PAD) Journal of Extracellular Vesicles 2020-09-01 [PMID: 32158521]
S Kijani, AM Vázquez, M Levin, J Borén, P Fogelstran Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis Physiol Rep, 2017-07-01;5(14):. 2017-07-01 [PMID: 28716818] (Functional Assay, Mouse) Functional Assay Mouse
Shirasaki T, Honda M, Shimakami T, Horii R, Yamashita T, Sakai Y, Sakai A, Okada H, Watanabe R, Murakami S, Yi M, Lemon S, Kaneko S MicroRNA-27a regulates lipid metabolism and inhibits hepatitis C virus replication in human hepatoma cells. J Virol, 2013-02-28;87(9):5270-86. 2013-02-28 [PMID: 23449803] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol APOB
Uniprot