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Apolipoprotein B/ApoB Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Apolipoprotein B/ApoB Antibody [NBP2-38608] - Staining of human cell line Hep G2 shows localization to cytosol & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NBP2-38608] - Staining of human prostate shows no positivity in glandular cells.
Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NBP2-38608] - Staining of human gastrointestinal shows moderate positivity in plasma in blood vessels.
Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NBP2-38608] - Staining of human lymphoid tissues shows no positivity in lymphoid cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Apolipoprotein B/ApoB Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: NFVASHIANILNSEELDIQDLKKLVKEALKESQLPTVMDFRKFSRNYQLYKSVSLPSLDPASAKIEGNLIFDPNNYLPKESMLKTTLTAFGFASADLIE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APOB
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Apolipoprotein B/ApoB Protein (NBP2-38608PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Apolipoprotein B/ApoB Antibody

  • Apo B-100
  • APOB
  • ApoB-100
  • ApoB-48
  • apolipoprotein B (including Ag(x) antigen)
  • Apolipoprotein B
  • apolipoprotein B-100
  • apolipoprotein B48
  • FLDB
  • LDLCQ4
  • mutant Apo B 100

Background

Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL). The molecular mass is 260 kDa. Apo B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol APOB
Uniprot