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Apolipoprotein B/ApoB Antibody

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Western Blot: Apolipoprotein B/ApoB Antibody [NB200-527] - Alcohol feeding significantly down-regulated ApoB expression in mouse liver. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Apolipoprotein B/ApoB Antibody Summary

Immunogen
Mouse low density lipoprotein (LDL)
Localization
Secreted
Specificity
Recognizes mouse as well as human Apo B48 and B100 as these proteins are highly conserved.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APOB
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
Use in immunoprecipitation reported in scientific literature (PMID:31807238).
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB200-527 in the following applications:

Publications
Read Publications using
NB200-527 in the following applications:

  • IP
    1 publication
  • WB
    4 publications

Reactivity Notes

Recognizes mouse as well as human Apo B48 and B100 as these proteins are highly conserved. Rat reactivity reported in scientific literature (PMID: 29964320).

Packaging, Storage & Formulations

Storage
Store at -70C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for Apolipoprotein B/ApoB Antibody

  • Apo B-100
  • APOB
  • ApoB-100
  • ApoB-48
  • apolipoprotein B (including Ag(x) antigen)
  • Apolipoprotein B
  • apolipoprotein B-100
  • apolipoprotein B48
  • FLDB
  • LDLCQ4
  • mutant Apo B 100

Background

Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549 kDa and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800mg/L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein B/ApoB Antibody (NB200-527)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(1)
WB
(4)
All Applications
Filter By Species
Human
(1)
Mouse
(3)
Rat
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB200-527 Applications Species
Liu W, Sun C, Yan Y Et al. Hepatic P38 Activation Modulates Systemic Metabolism Through Fgf21-Mediated Interorgan Communication Diabetes 2021-10-21 [PMID: 34675006]
Matsumoto A, Takahashi Y, Ogata K Et Al. Phosphatidylserine-deficient small extracellular vesicle is a major somatic cell-derived sEV subpopulation in blood iScience 2021-08-01 [PMID: 34368655]
Song Y, Kim S, Lee H et al. Chromenopyrimidinone Controls Stemness and Malignancy by suppressing CD133 Expression in Hepatocellular Carcinoma Cancers (Basel) 2020-05-08 [PMID: 32397206] (WB, Human) WB Human
Matsumoto A, Takahashi Y, Chang H et al. Blood concentrations of small extracellular vesicles are determined by a balance between abundant secretion and rapid clearance J Extracell Vesicles 2019-01-01 [PMID: 31807238] (IP, Mouse) IP Mouse
Howell GE, McDevitt E, Henein L et al. Alterations in cellular lipid metabolism produce neutral lipid accumulation following exposure to the organochlorine compound trans-nonachlor in rat primary hepatocytes. Environ. Toxicol. 2018-07-02 [PMID: 29964320] (WB, Rat) WB Rat
Grefhorst A, van den Beukel JC, Dijk W et al. Multiple effects of cold exposure on livers of male mice J. Endocrinol. 2018-05-09 [PMID: 29743343] (WB, Mouse) WB Mouse
Tsai WC, Hsu SD, Hsu CS et al. MicroRNA-122 plays a critical role in liver homeostasis and hepatocarcinogenesis J Clin Invest 2012-08-01 [PMID: 22820290] (WB, Mouse) WB Mouse

Reviews for Apolipoprotein B/ApoB Antibody (NB200-527) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB200-527:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Apolipoprotein B/ApoB NB200-527
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 03/01/2018
View

Summary

ApplicationWestern Blot
Sample TestedLiver
SpeciesMouse
Lot2c08910
Western Blot Apolipoprotein B/ApoB NB200-527
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 04/05/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse Liver Tissue
SpeciesMouse

Product General Protocols

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Video Protocols

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FAQs for Apolipoprotein B/ApoB Antibody (NB200-527) (0)

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Verified Customer
03/01/2018
Application: WB
Species: Mouse

Verified Customer
04/05/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol APOB
Entrez