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Apolipoprotein A-I/ApoA1 Antibody

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Immunocytochemistry/ Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apolipoprotein A1 stained by Apolipoprotein A1 antibody ...read more
Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] - Human ovarian cancer. Apolipoprotein A1 stained by Apolipoprotein A1 antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, ...read more
Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] - Human plasma (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody diluted at 1:10000. HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] -HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apolipoprotein A1 stained by Apolipoprotein A1 antibody ...read more
Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] - Mouse plasma and rat plasma (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody diluted at 1:10000. The ...read more
Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] -Human colon. Apolipoprotein A1 stained by Apolipoprotein A1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] - Human lung. Apolipoprotein A1 stained by Apolipoprotein A1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] - Apolipoprotein A-I/ApoA1 antibody detects secreted Apolipoprotein A-I/ApoA1 protein by immunohistochemical analysis.Sample: ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, SyHaSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Apolipoprotein A-I/ApoA1 Antibody Summary

Immunogen
Full length human Apolipoprotein A-I/ApoA1 Recombinant protein.
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APOA1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:20000
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-15429 in the following applications:

  • WB
    1 publication

Reactivity Notes

Pig (80%). Use in Golden Syrian Hamster reported in scientific literature (PMID:36110841).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for Apolipoprotein A-I/ApoA1 Antibody

  • Alp-1
  • APOA1
  • Apo-AI
  • apoA-I
  • Apolipoprotein A1
  • Apolipoprotein AI
  • Apolipoprotein A-I
  • Brp-14
  • Ltw-1
  • Lvtw-1
  • MGC117399
  • Sep-1
  • Sep-2

Background

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein A-I/ApoA1 Antibody (NBP2-15429)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol APOA1
Uniprot