Home » Apolipoprotein A-I/ApoA1 » Apolipoprotein A-I/ApoA1 Antibodies » Apolipoprotein A-I/ApoA1 Antibody (2083A) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

Apolipoprotein A-I/ApoA1 Antibody (2083A) [Unconjugated]

 Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Apolipoprotein A-I/ApoA1 Monoclonal Antibody (Catalog # MAB36641) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Apolipoprotein A-I/ApoA1 ...read more
HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Apolipoprotein A-I/ApoA1 Monoclonal Antibody (Catalog # MAB36641, filled histogram) or isotype control antibody (Catalog # MAB1050, open ...read more
Apolipoprotein A-I/ApoA1 was detected in immersion fixed human hepatocytes using Rabbit Anti-Human Apolipoprotein A-I/ApoA1 Monoclonal Antibody (Catalog # MAB36641) at 1 µg/mL for 3 hours at room temperature. Cells ...read more
Apolipoprotein A-I/ApoA1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Rabbit Anti-Human Apolipoprotein A-I/ApoA1 Monoclonal Antibody (Catalog # MAB36641) at 1 µg/mL for 3 hours ...read more
Apolipoprotein A‑I/ApoA1 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Rabbit Anti-Human Apolipoprotein A‑I/ApoA1 Monoclonal Antibody (Catalog # MAB36641) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, Flow, ICC/IF
Clone
2083A
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB36641U-100UG
Alexa Fluor 405
FAB36641V-100UG
Alexa Fluor 488
FAB36641G-100UG
Alexa Fluor 532
FAB36641X
Alexa Fluor 594
FAB36641T-100UG
Alexa Fluor 647
FAB36641R-100UG
Alexa Fluor 700
FAB36641N-100UG
Alexa Fluor 750
FAB36641S-100UG
Allophycocyanin
FAB36641A
Biotin
FAB36641B
CoraFluor 1
FAB36641CL1
DyLight 350
FAB36641D
DyLight 405
FAB36641E
DyLight 488
FAB36641K
DyLight 550
FAB36641L
DyLight 594
FAB36641M
DyLight 650
FAB36641W
DyLight 680
FAB36641Y
DyLight 755
FAB36641Z
FITC
FAB36641F
HRP
FAB36641H
Janelia Fluor 525
FAB36641JF525
Janelia Fluor 549
FAB36641I
Janelia Fluor 646
FAB36641J
Janelia Fluor 669
FAB36641JF669
PE
FAB36641P
PerCP
FAB36641C
mFluor Violet 450 SE
FAB36641MFV450
mFluor Violet 500 SE
FAB36641MFV500
mFluor Violet 610 SE
FAB36641MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB36641-SP
Carrier Free
FAB36641V-100UG
Carrier Free
FAB36641U-100UG
Carrier Free
FAB36641T-100UG
Carrier Free
FAB36641S-100UG
Carrier Free
FAB36641R-100UG
Carrier Free
FAB36641N-100UG
Carrier Free
FAB36641G-100UG
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Apolipoprotein A-I/ApoA1 Antibody (2083A) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
E. coli-derived recombinant human ApoA1
Asp25-Gln267
Accession # P02647
Specificity
Detects human ApoA1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 1-25 ug/mL
  • Immunohistochemistry 1-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Apolipoprotein A-I/ApoA1 Antibody (2083A) [Unconjugated]

  • Alp-1
  • APOA1
  • Apo-AI
  • apoA-I
  • Apolipoprotein A1
  • Apolipoprotein AI
  • Apolipoprotein A-I
  • Brp-14
  • Ltw-1
  • Lvtw-1
  • MGC117399
  • Sep-1
  • Sep-2

Background

ApoA1 is a major protein component of serum high-density lipoprotein (HDL) and is produced by the liver and small intestine. It is involved in reverse cholesterol transport from tissues to the liver. ApoA1 is synthesized as a 267 amino acid (aa) precursor from which a signal peptide and short propeptide are removed. Mature human ApoA1 shares 65% and 61% aa sequence identity with mouse and rat ApoA1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Apolipoprotein
A-II/ApoA2 Antibody ( ...
NBP3-16168
Immunohistochemistry Apolipoprotein A-II/ApoA2 Antibody (5F7U1)
Western Blot Apolipoprotein A-II/ApoA2 Antibody (5F7U1)
Immunohistochemistry Apolipoprotein A-II/ApoA2 Antibody (5F7U1)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, WB
ABCA1 Antibody -
BSA Free
NB400-105
Dual RNAscope ISH-IHC ABCA1 Antibody - BSA Free
Immunohistochemistry-Paraffin ABCA1 Antibody - BSA Free
Western Blot ABCA1 Antibody - BSA Free
(7 Reviews)
(408 Publications)
Species: Ca, Ch, ChHa, Eq, Ha, Hu, Mu, Md, Po, Pm, Rb, Rt
Applications: B/N, ChIP, ChIP, Dual ISH-IHC, ELISA, Flow, GS, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, PCR, Simple Western, WB
Apolipoprotein
CIII Antibody
NB600-610
Immunohistochemistry Apolipoprotein CIII Antibody
Immunohistochemistry-Paraffin Apolipoprotein CIII Antibody
(1 Review)
(2 Publications)
Species: Bv, Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
CETP Antibody
(ATM192) - BSA Free
NB300-558
Immunocytochemistry/ Immunofluorescence CETP Antibody (ATM192) - BSA Free
Western Blot CETP Antibody (ATM192) - BSA Free
(1 Publication)
Species: Bv, Hu, Rb
Applications: ELISA, ICC/IF, IP, WB
LCAT Antibody
NBP1-05950
Western Blot LCAT Antibody
Immunocytochemistry/ Immunofluorescence LCAT Antibody
(1 Review)
(3 Publications)
Species: Hu, Mu
Applications: ICC/IF, WB
Lipoprotein a
Antibody (4H1) - BSA
NBP2-37477
ELISA Lipoprotein a Antibody (4H1) - BSA Free
Western Blot Lipoprotein a Antibody (4H1) - BSA Free
Immunocytochemistry/ Immunofluorescence Lipoprotein a Antibody (4H1) - BSA Free
(2 Publications)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Thyroglobulin
Antibody (2H11 + 6E1)
NBP2-34294
Immunohistochemistry-Paraffin Thyroglobulin Antibody (2H11 + 6E1)
Immunohistochemistry Thyroglobulin Antibody (2H11 + 6E1)
Immunohistochemistry-Paraffin Thyroglobulin Antibody (2H11 + 6E1)
(3 Reviews)
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P
Apolipoprotein
A-IV/ApoA4 Antibody ( ...
NBP2-52932
Western Blot Apolipoprotein A-IV/ApoA4 Antibody (CL0468)
Immunohistochemistry-Paraffin Apolipoprotein A-IV/ApoA4 Antibody (CL0468)
Immunohistochemistry-Paraffin Apolipoprotein A-IV/ApoA4 Antibody (CL0468)
(1 Review)
Species: Hu
Applications: IHC, IHC-P, WB
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(24 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
Apolipoprotein
E/ApoE Antibody (WUE- ...
NB110-60531
Flow (Intracellular) Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free
Western Blot Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free
Flow Cytometry Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free
(2 Reviews)
(38 Publications)
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
Apolipoprotein
B/ApoB Antibody (3697 ...
MAB4124
Immunohistochemistry Apolipoprotein B/ApoB Antibody (369717) [Unconjugated]
Western Blot Apolipoprotein B/ApoB Antibody (369717) [Unconjugated]
Simple Western Apolipoprotein B/ApoB Antibody (369717) [Unconjugated]
(7 Publications)
Species: Hu
Applications: IHC, Simple Western, WB
Albumin Antibody
(188835) [Unconjugate ...
MAB1455
Simple Western Albumin Antibody (188835) [Unconjugated] - Serum
Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum
Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum
(3 Reviews)
(54 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
Lipoprotein
Lipase/LPL Antibody [ ...
AF7197
Immunohistochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]
Western Blot Lipoprotein Lipase/LPL Antibody [Unconjugated]
Immunocytochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]
(12 Publications)
Species: Hu, Mu
Applications: ICC, IHC, WB
SR-BI Antibody -
BSA Free
NB400-104
Immunohistochemistry-Paraffin SR-BI Antibody - BSA Free
Knockdown Validated SR-BI Antibody - BSA Free
Western Blot SR-BI Antibody - BSA Free
(5 Reviews)
(234 Publications)
Species: ChHa, SyHa, Ha, Hu, Mu, Md, Po, Pm, Rb, Rt
Applications: B/N, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, PLA, Simple Western, WB
CD55/DAF Antibody
[Unconjugated]
AF2009
Immunohistochemistry CD55/DAF Antibody [Unconjugated]
Immunocytochemistry CD55/DAF Antibody [Unconjugated]
(8 Publications)
Species: Hu
Applications: ICC, IHC
Transcobalamin II
Antibody
AF7895
Western Blot Transcobalamin II Antibody
Immunohistochemistry Transcobalamin II Antibody
Species: Hu
Applications: IHC, WB

Publications for Apolipoprotein A-I/ApoA1 Antibody (MAB36641) (0)

There are no publications for Apolipoprotein A-I/ApoA1 Antibody (MAB36641).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Apolipoprotein A-I/ApoA1 Antibody (MAB36641) (0)

There are no reviews for Apolipoprotein A-I/ApoA1 Antibody (MAB36641). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Apolipoprotein A-I/ApoA1 Antibody (MAB36641) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Apolipoprotein A-I/ApoA1 Products

Blogs on Apolipoprotein A-I/ApoA1

There are no specific blogs for Apolipoprotein A-I/ApoA1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Klotho beta Monoclonal Antibody (Catalog # MAB58891, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.
Rabbit IgG Isotype Control [Unconjugated]
AB-105-C
Formalin-fixed paraffin-embedded tissue sections of human prostate cancer were probed for ABCA1 mRNA (ACD RNAScope Probe, catalog # 432291; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus Biologicals catalog # NB400-105) at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.
ABCA1 Antibody - BSA Free
NB400-105

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Apolipoprotein A-I/ApoA1 Antibody (2083A) [Unconjugated] and receive a gift card or discount.


Bioinformatics

Uniprot