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Angiopoietin-like Protein 6/ANGPTL6 Antibody (766544) [Unconjugated]

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Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, 3T3-L1 mouse embryonic fibroblast adipose-like cell line, and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF
Clone
766544
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

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Angiopoietin-like Protein 6/ANGPTL6 Antibody (766544) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse Angiopoietin-like Protein 6/ANGPTL6
Ala25-Leu457
Accession # Q8ROZ6
Specificity
Detects mouse Angiopoietin-like Protein 6/ANGPTL6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Angiopoietin-like 6 or recombinant mouse Angiopoietin-like 2 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
ANGPTL6
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publication using MAB7527.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-like Protein 6/ANGPTL6 Antibody (766544) [Unconjugated]

  • AGF
  • AGFAngiopoietin-related protein 5
  • Angiopoietin like Protein 6
  • angiopoietin-like 6
  • Angiopoietin-like Protein 6
  • Angiopoietin-related growth factor
  • ANGPTL6
  • ARP5
  • ARP5angiopoietin-related protein 6

Background

Angiopoietin-like 6 (ANGPTL6), also known as angiopoietin-related growth factor (AGF), is a secreted 50 kDa protein that contains a coiled-coil domain (aa 51-77,
126-164) and a fibrinogen-like domain (aa 238-456). A conserved Integrin-binding RGD motif is located within the fibrinogen-like domain. This enables ANGPTL6 to promote skin wound healing by mediating the adhesion and migration of keratinocytes, fibroblasts, and endothelial cells. ANGPTL6 also promotes the chemotaxis of vascular endothelial cells resulting in increased vascular permeability and angiogenesis. ANGPTL6 is also secreted by hepatocytes. It inhibits gluconeogenesis in these cells and promotes insulin sensitivity and energy expenditure in mice fed high fat diets. Serum levels of ANGPTL6 are elevated in metabolic syndrome, diabetes, and preeclampsia but are decreased in chronic renal failure. ANGPTL6 is additionally produced by several hematopoietic cell types including megakaryocytes, platelets, mast cells, and uterine NK cells. Mature mouse ANGPTL6 shares 75% and 95% sequence identity with human and rat ANGPTL6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ANGPTL6
Uniprot