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Androgen R/NR3C4 Antibody (2621E) [Unconjugated]

 
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Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58762) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Androgen R/NR3C4 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58762) followed by HRP-conjugated ...read more
Androgen R/NR3C4 was detected in immersion fixed LNCaP human prostate cancer cell line (left panel; positive staining) and PC‑3 human prostate cancer cell line (right panel; negative staining) using Rabbit ...read more
Androgen R/NR3C4 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using Rabbit Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58762) at 3 µg/mL for ...read more
LNCaP human prostate cancer cell line was stained with Rabbit Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58762, filled histogram) or isotype control antibody (MAB1050, open histogram), followed by ...read more
Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Androgen R/NR3C4 at approximately 121 kDa (as indicated) using 10 µg/mL of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, CyTOF-ready, Flow, ICC/IF
Clone
2621E
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC58762U-100UG
Alexa Fluor 405
IC58762V-100UG
Alexa Fluor 488
IC58762G-100UG
Alexa Fluor 532
FAB58762X
Alexa Fluor 594
IC58762T-100UG
Alexa Fluor 647
IC58762R-100UG
Alexa Fluor 700
IC58762N-100UG
Alexa Fluor 750
IC58762S-100UG
Allophycocyanin
FAB58762A
Biotin
FAB58762B
DyLight 350
FAB58762D
DyLight 405
FAB58762E
DyLight 488
FAB58762K
DyLight 550
FAB58762L
DyLight 594
FAB58762M
DyLight 650
FAB58762W
DyLight 680
FAB58762Y
DyLight 755
FAB58762Z
FITC
FAB58762F
HRP
FAB58762H
Janelia Fluor 549
FAB58762I
Janelia Fluor 646
FAB58762J
PE
FAB58762P
PerCP
FAB58762C
mFluor Violet 450 SE
FAB58762MFV450
mFluor Violet 500 SE
FAB58762MFV500
mFluor Violet 610 SE
FAB58762MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB58762-SP
Carrier Free
IC58762V-100UG
Carrier Free
IC58762U-100UG
Carrier Free
IC58762T-100UG
Carrier Free
IC58762S-100UG
Carrier Free
IC58762R-100UG
Carrier Free
IC58762N-100UG
Carrier Free
IC58762G-100UG
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Androgen R/NR3C4 Antibody (2621E) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic Human Androgen R/NR3C4 peptide
Specificity
Detects human Androgen R/NR3C4 in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Androgen R/NR3C4 Antibody (2621E) [Unconjugated]

  • AIS
  • Androgen R
  • androgen receptor
  • AndrogenR
  • AR
  • DHTR
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • NR3C4
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SBMA
  • SMAX1
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Background

The Androgen Receptor (AR) is a 90 kDa steroid hormone receptor that is critical for the development and function of the male reproductive system. AR binding to testosterone or 5 alpha -dihydrotestosterone (DHT) triggers receptor dimerization followed by translocation to the nucleus where it promotes transcription of androgen responsive genes. Multiple polymorphisms in AR are linked to the development of prostate cancer. The ligand binding domain of human AR (aa 661-920) shares 100% aa sequence identity with mouse and rat AR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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