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AMBRA1 Antibody [DyLight 550]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 550

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-07124AF350
Alexa Fluor 405
NBP1-07124AF405
Alexa Fluor 488
NBP1-07124AF488
Alexa Fluor 532
NBP1-07124AF532
Alexa Fluor 594
NBP1-07124AF594
Alexa Fluor 647
NBP1-07124AF647
Alexa Fluor 700
NBP1-07124AF700
Alexa Fluor 750
NBP1-07124AF750
Allophycocyanin
NBP1-07124APC
Biotin
NBP1-07124B
DyLight 350
NBP1-07124UV
DyLight 405
NBP1-07124V
DyLight 488
NBP1-07124G
DyLight 594
NBP1-07124DL594
DyLight 650
NBP1-07124C
DyLight 680
NBP1-07124FR
DyLight 755
NBP1-07124IR
FITC
NBP1-07124F
HRP
NBP1-07124H
Janelia Fluor 525
NBP1-07124JF525
Janelia Fluor 549
NBP1-07124JF549
Janelia Fluor 646
NBP1-07124JF646
Janelia Fluor 669
NBP1-07124JF669
PE
NBP1-07124PE
PerCP
NBP1-07124PCP
Unconjugated
NBP1-07124
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-07124
BSA Free
NBP1-07124
BSA Free
NBP1-07124SS
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AMBRA1 Antibody [DyLight 550] Summary

Immunogen
A synthetic peptide derived from mouse activating molecule in Beclin 1-regulated/AMBRA1 protein sequence [amino acids range 1175-1250] [UniProt # A2AH22]
Localization
Cytoplasmic vesicle, autophagosome.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AMBRA1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for AMBRA1 Antibody [DyLight 550]

  • activating molecule in BECN1-regulated autophagy protein 1
  • autophagy/beclin-1 regulator 1
  • DCAF3
  • DDB1 and CUL4 associated factor 3
  • FLJ20294
  • KIAA1736activating molecule in beclin-1-regulated autophagy
  • MGC33725
  • WD repeat domain 94
  • WDR94

Background

AMBRA1 (Activating molecule in Beclin1-regulated autophagy protein 1, Kiaa1736, DCAF3) is a regulator of autophagy and neural development in embryogenesis. Ambra1 controls autophagy in the central nervous system by activating class III PtdIns3k complexes that are required for phagophore nucleation. Beclin1 and Vps34 interaction are promoted by AMBRA1, and help form this complex. The loss of AMBRA1 causes upregulated apoptosis and accumulation of ubiquitinated proteins, which finally proves lethal. It was recently discovered that AMBRA1 interacts with Parkin after prolonged mitochondrial depolarization, thus triggering mitophagy (PMID 21921694). AMBRA1 has 7 known isoforms, and numerous WD40 domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for AMBRA1 Antibody (NBP1-07124R)

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Blogs on AMBRA1.

Check out the latest blog posts on AMBRA1.
Optogenetic Control of Mitophagy: AMBRA1 based mitophagy switch
By Christina Towers, PhD Mitophagy in the BrainSelective autophagic degradation of damaged mitochondria, known as mitophagy, has been described as a cyto-protective process. Accordingly, defects in mitophagy h...  Read full blog post.
Animal Models to Study Autophagy
By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.
Beclin 2, a mammal-specific homolog of Beclin 1 with unique functional similarities and differences
Beclin 2 (BECN2) is also called Beclin-1-like protein 1/ BECN1P1 and it was recently identified by He et al 2013 as a mammal-specific homolog of the evolutionarily conserved protein Beclin 1 which is well established for its role in the regulation ...  Read full blog post.
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Bioinformatics

Gene Symbol AMBRA1