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AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1)

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Western Blot: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Western Blot Analysis of HeLa cell lysate using AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).
Western Blot: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Western Blot Analysis of HeLa cell lysate using AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Formalin-fixed, paraffin-embedded Rat Heart Muscle stained with AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody ...read more
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Formalin-fixed, paraffin-embedded human Breast stained with AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).
Protein Array: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Analysis of Protein Array containing more than 19,000 full-length human proteins using AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, MA
Clone
CPTC-CRYAB-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08380AF350
Alexa Fluor 405
NBP3-08380AF405
Alexa Fluor 488
NBP3-08380AF488
Alexa Fluor 532
NBP3-08380AF532
Alexa Fluor 594
NBP3-08380AF594
Alexa Fluor 647
NBP3-08380AF647
Alexa Fluor 700
NBP3-08380AF700
Alexa Fluor 750
NBP3-08380AF750
Allophycocyanin
NBP3-08380APC
Biotin
NBP3-08380B
CoraFluor 1
NBP3-08380CL1
DyLight 350
NBP3-08380UV
DyLight 405
NBP3-08380V
DyLight 488
NBP3-08380G
DyLight 550
NBP3-08380R
DyLight 594
NBP3-08380DL594
DyLight 650
NBP3-08380C
DyLight 680
NBP3-08380FR
DyLight 755
NBP3-08380IR
FITC
NBP3-08380F
HRP
NBP3-08380H
Janelia Fluor 525
NBP3-08380JF525
Janelia Fluor 549
NBP3-08380JF549
Janelia Fluor 585
NBP3-08380JF585
Janelia Fluor 635
NBP3-08380JF635
Janelia Fluor 646
NBP3-08380JF646
Janelia Fluor 669
NBP3-08380JF669
PE
NBP3-08380PE
PerCP
NBP3-08380PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-08380
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AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08380)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant human full-length AlphaB Crystallin/CRYAB protein (Uniprot: P02511)
Localization
Cytoplasm. Translocates to nucleus during heat shock and resides in nuclear splicing speckles.
Isotype
IgG2c Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CRYAB
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1)

  • alpha B crystallin
  • alpha(B)-crystallin
  • AlphaB Crystallin
  • alpha-crystallin B chain
  • CRYA2
  • CRYA2alpha crystallin B chain
  • CRYAB
  • crystallin, alpha B
  • CTPP2
  • Heat shock protein beta-5
  • heat-shock 20 kD like-protein
  • HSPB5
  • Renal carcinoma antigen NY-REN-27
  • Rosenthal fiber component

Background

Crystallin AB is a member of the small heat shock protein family and expressed in several tissues including brain, kidney cardiac and skeletal muscle. Crystallin AB functions as a molecular chaperone and in intracellular architecture. Crystallin AB has shown to have interactions with CRYAA, CRYGC, CRYBB2, CRYBA1 and HSPB8. Defects in crystalline AB have been linked to cause myopathy myofibrillar type 2, cataract posterior polar type 2, and myopathy myofibrillar fatal infantile hypertonic alpha-B crystallin-related (MFMFIH-CRYAB).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CRYAB
Uniprot