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alpha-Synuclein Antibody [Unconjugated]

Images

 
Western blot shows lysates of human brain (motor cortex) tissue, human brain (cerebellum) tissue, SH-SY5Y human neuroblastoma cell line, and U-87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed ...read more
a-Synuclein was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Goat Anti-Human a-Synuclein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1338) at 15 µg/mL ...read more
Recombinant Human a-Synuclein protein was serially diluted 2-fold and captured by Mouse Anti-Human a-Synuclein Monoclonal Antibody (MAB13384) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human a-Synuclein ...read more
Simple Western lane view shows lysates of SK‑Mel‑28 human malignant melanoma cell line, loaded at 0.2 mg/ml. A specific band was detected for alpha -Synuclein at approximately 21 kDa (as indicated) using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price
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alpha-Synuclein Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human alpha -Synuclein
Met1-Ala140
Accession # P37840
Specificity
Detects human alpha -Synuclein in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh)  beta -Synuclein and rh gamma -Synuclein is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SNCA
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF1338 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Synuclein Antibody [Unconjugated]

  • alpha-Synuclein
  • I+/--synuclein
  • MGC110988
  • NACP
  • non A-beta component of AD amyloid
  • Non-A beta component of AD amyloid
  • Non-A4 component of amyloid precursor
  • PARK1
  • PARK4
  • PD1
  • SNCA
  • synuclein alpha-140
  • synuclein, alpha (non A4 component of amyloid precursor)
  • Synuclein-alpha
  • truncated alpha synuclein

Background

alpha -Synuclein is a cytoplasmic protein that is predominantly expressed in the central nervous system. It is a minor protein found in amyloid deposition that is a hallmark of Alzheimer's disease. Defects in SNCA are associated with familial Parkinson's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Synuclein Antibody (AF1338)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC, IHC-P, Western Blot.


Filter By Application
ICC
(1)
IHC-P
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(2)
All Species

Reviews for alpha-Synuclein Antibody (AF1338) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for alpha-Synuclein Antibody (AF1338). (Showing 1 - 1 of 1 FAQs).

  1. I'm looking for an alpha-Synuclein antibody with an epitope located in the first half (N-terminus) of the protein - preferably a monoclonal antibody. Can you help me with that?
    • Please take a look at NB110-57475. It has been validated for human, rat and mouse and the applications ICC and WB and the epitope it detects is in the N terminal.

Secondary Antibodies

 

Isotype Controls

Additional alpha-Synuclein Products

Blogs on alpha-Synuclein.

Mechanisms of Neurodegeneration: Mitochondrial Dysfunction and Oxidative Stress
By Michalina Hanzel, PhDIn this second installment of our three blog-posts series on major cellular mechanisms responsible for neurodegenerative disorders, we will explore the processes of mitochondrial dysfunction an...  Read full blog post.

Mechanisms of Neurodegeneration: Protein aggregation and failure of autophagy
By Michalina Hanzel, PhDIn a series of three blog posts I will briefly explore the major cellular mechanisms responsible for many neurodegenerative disorders. The first, and perhaps the most apparent, is the accumulat...  Read full blog post.

The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation
The root of Parkinson’s disease (PD) points to a poorly regulated electron transport chain leading to mitochondrial damage, where many proteins need to work cohesively to ensure proper function.  The two key players of this pathway are PINK1, ...  Read full blog post.

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Bioinformatics

Gene Symbol SNCA
Uniprot