![Western Blot: alpha Glucosidase 2 Antibody [NBP1-32554] - Wild-type (WT) and alpha Glucosidase II knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Glucosidase II antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Western-Blot-NBP1-32554-img0011.jpg "Western Blot: alpha Glucosidase 2 Antibody [NBP1-32554] - Wild-type (WT) and alpha Glucosidase II knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Glucosidase II antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human alpha Glucosidase 2. The exact sequence is proprietary. |
| Localization | Endoplasmic reticulum , Golgi apparatus , Melanosome |
| Predicted Species | Rhesus Macaque (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GANAB |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 107 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: alpha Glucosidase 2 Antibody [NBP1-32554] - Analysis of methanol-fixed HeLa, using alpha Glucosidase II antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32554-img0006.jpg "Immunocytochemistry/Immunofluorescence: alpha Glucosidase 2 Antibody [NBP1-32554] - Analysis of methanol-fixed HeLa, using alpha Glucosidase II antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.")
![Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-32554] - Human breast carcinoma. alpha Glucosidase II antibody [N1N2], N-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-32554-img0010.jpg "Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-32554] - Human breast carcinoma. alpha Glucosidase II antibody [N1N2], N-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: alpha Glucosidase 2 Antibody [NBP1-32554] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Western-Blot-NBP1-32554-img0008.jpg "Western Blot: alpha Glucosidase 2 Antibody [NBP1-32554] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Western Blot: alpha Glucosidase 2 Antibody [NBP1-32554] - Sample(30 ug whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 7.5% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Western-Blot-NBP1-32554-img0009.jpg "Western Blot: alpha Glucosidase 2 Antibody [NBP1-32554] - Sample(30 ug whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 7.5% SDS PAGE diluted at 1:1000.")
![Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-32554] - SW480 xenograft , using alpha Glucosidase II antibody at 1:500 dilution.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-32554-img0007.jpg "Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-32554] - SW480 xenograft , using alpha Glucosidase II antibody at 1:500 dilution.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")