alpha Glucosidase 2 Antibodies
We offer alpha Glucosidase 2 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each alpha Glucosidase 2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our alpha Glucosidase 2 Antibodies can be used in a variety of model species: Bovine, Canine, Guinea Pig, Human, Mouse, Primate, Rabbit, Sheep. Use the list below to choose the alpha Glucosidase 2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our alpha Glucosidase 2 polyclonal antibodies.
Alternate Names for alpha Glucosidase 2 Antibodies
anti-alpha Glucosidase 2 antibody, anti-GANAB antibody, anti-Alpha-glucosidase 2 antibody, anti-EC 3.2.1 antibody, anti-EC 3.2.1.84 antibody, anti-G2ANGLUII antibody, anti-Glucosidase II subunit alpha antibody, anti-glucosidase, alpha; neutral AB antibody, anti-GluII antibody, anti-KIAA0088alpha glucosidase II alpha subunit antibody, anti-neutral alpha-glucosidase AB antibody
9 Results for "alpha-glucosidase-2" in Primary Antibodies
![Western Blot: alpha Glucosidase 2 Antibody [NBP1-32554] - Wild-type (WT) and alpha Glucosidase II knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Glucosidase II antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Western-Blot-NBP1-32554-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: alpha Glucosidase 2 Antibody [NBP1-32554] - Wild-type (WT) and alpha Glucosidase II knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Glucosidase II antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: alpha Glucosidase 2 Antibody [NBP1-32554] - Analysis of methanol-fixed HeLa, using alpha Glucosidase II antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32554-img0006.jpg)
![Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-32554] - Human breast carcinoma. alpha Glucosidase II antibody [N1N2], N-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-32554-img0010.jpg)
![Western Blot: alpha Glucosidase 2 Antibody [NBP1-32554] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Western-Blot-NBP1-32554-img0008.jpg)
![Western Blot: alpha Glucosidase 2 Antibody [NBP1-32554] - Sample(30 ug whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 7.5% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Western-Blot-NBP1-32554-img0009.jpg)
![Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-32554] - SW480 xenograft , using alpha Glucosidase II antibody at 1:500 dilution.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-32554-img0007.jpg)
![Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP2-49332] - Staining in human thyroid gland and skeletal muscle tissues using anti-GANAB antibody. Corresponding GANAB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-49332-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP2-49332] - Staining in human thyroid gland and skeletal muscle tissues using anti-GANAB antibody. Corresponding GANAB RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP2-49332] - Staining of human cerebral cortex, colon, skeletal muscle and thyroid gland using Anti-GANAB antibody NBP2-49332 (A) shows similar protein distribution across tissues to independent antibody NBP1-89806 (B).](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-49332-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Glucosidase 2 Antibody [NBP2-49332] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49332-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP2-49332] - Staining of human colon.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-49332-img0010.jpg)
![Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP2-49332] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-49332-img0005.jpg)
![Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP2-49332] - Staining of human thyroid gland shows high expression.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-49332-img0006.jpg)
![Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP2-49332] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-49332-img0009.jpg)
![Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-89806] - Staining of human colon.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-89806-img0012.jpg "Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-89806] - Staining of human colon.")
![Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-89806] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-89806-img0002.jpg)
![Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-89806] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-89806-img0007.jpg)
![Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-89806] - Staining of human thyroid gland shows high expression.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-89806-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-89806] - Staining in human thyroid gland and skeletal muscle tissues using anti-GANAB antibody. Corresponding GANAB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-89806-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-89806] - Staining of human cerebral cortex, colon, skeletal muscle and thyroid gland using Anti-GANAB antibody NBP1-89806 (A) shows similar protein distribution across tissues to independent antibody NBP2-49332 (B).](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-89806-img0010.jpg)
![Immunohistochemistry-Paraffin: alpha Glucosidase 2 Antibody [NBP1-89806] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-89806-img0011.jpg)
![Immunoprecipitation: alpha Glucosidase 2 Antibody (PSH01-52) [NBP3-32366] - alpha Glucosidase 2 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32366 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32366 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32366 in HeLa cell lysate Lane 3: NBP3-32366 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 21 seconds](http://images.novusbio.com/fullsize/nbp3-32366_rabbit-alpha-glucosidase-2-mab-psh01-52-26820241582613.jpg "Immunoprecipitation: alpha Glucosidase 2 Antibody (PSH01-52) [NBP3-32366] - alpha Glucosidase 2 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32366 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32366 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32366 in HeLa cell lysate Lane 3: NBP3-32366 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 21 seconds")
![Immunohistochemistry: alpha Glucosidase 2 Antibody (PSH01-52) [NBP3-32366] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-alpha Glucosidase 2 antibody (NBP3-32366) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32366) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32366_rabbit-alpha-glucosidase-2-mab-psh01-52-29820241435271.jpg)
![Immunohistochemistry: alpha Glucosidase 2 Antibody (PSH01-52) [NBP3-32366] - Immunofluorescence analysis of paraffin-embedded human thyroid tissue labeling alpha Glucosidase 2 with Rabbit anti-alpha Glucosidase 2 antibody (NBP3-32366) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32366, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32366_rabbit-alpha-glucosidase-2-mab-psh01-52-298202414161829.jpg)
![Western Blot: alpha Glucosidase 2 Antibody (PSH01-52) [NBP3-32366] - Western blot analysis of alpha Glucosidase 2 on different lysates with Rabbit anti-alpha Glucosidase 2 antibody (NBP3-32366) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: HEK-293 cell lysate Lane 4: Raji cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C2C12 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 107 kDa Observed band size: 107 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32366_rabbit-alpha-glucosidase-2-mab-psh01-52-3920248453531.jpg)
![Western Blot: alpha Glucosidase 2 Antibody [H00023193-D01P] - Analysis of GANAB expression in mouse spleen.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Western-Blot-H00023193-D01P-img0004.jpg "Western Blot: alpha Glucosidase 2 Antibody [H00023193-D01P] - Analysis of GANAB expression in mouse spleen.")
![Immunocytochemistry/Immunofluorescence: alpha Glucosidase 2 Antibody [H00023193-D01P] - Analysis of antibody to GANAB on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Immunocytochemistry-Immunofluorescence-H00023193-D01P-img0001.jpg)
![Western Blot: alpha Glucosidase 2 Antibody [H00023193-D01P] - Analysis of GANAB expression in HeLa.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Western-Blot-H00023193-D01P-img0002.jpg)
![Western Blot: alpha Glucosidase 2 Antibody [H00023193-D01P] - Analysis of GANAB expression in human placenta.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Western-Blot-H00023193-D01P-img0003.jpg)
![Western Blot: alpha Glucosidase 2 Antibody [H00023193-D01P] - Analysis of GANAB expression in transfected 293T cell line by GANAB polyclonal antibody.Lane 1: GANAB transfected lysate(106.90 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Western-Blot-H00023193-D01P-img0005.jpg)
![Western Blot: alpha Glucosidase 2 Antibody [NBP3-35393] - Western blot analysis of various lysates using alpha Glucosidase 2 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35393_rabbit-alpha-glucosidase-2-pab-912202414112875.jpg "Western Blot: alpha Glucosidase 2 Antibody [NBP3-35393] - Western blot analysis of various lysates using alpha Glucosidase 2 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: alpha Glucosidase 2 Antibody [NBP3-35393] - Immunofluorescence analysis of U-2 OS cells using alpha Glucosidase 2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35393_rabbit-alpha-glucosidase-2-pab-912202414163048.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Glucosidase 2 Antibody [NBP3-35393] - Immunofluorescence analysis of NIH-3T3 cells using alpha Glucosidase 2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35393_rabbit-alpha-glucosidase-2-pab-912202414163093.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Glucosidase 2 Antibody [NBP3-35393] - Immunofluorescence analysis of C6 cells using alpha Glucosidase 2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35393_rabbit-alpha-glucosidase-2-pab-91220241581256.jpg)
![Western Blot: alpha Glucosidase 2 Antibody (PSH01-53) [NBP3-32367] - Western blot analysis of alpha Glucosidase 2 on different lysates with Rabbit anti-alpha Glucosidase 2 antibody (NBP3-32367) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: LoVo cell lysate (20 ug/Lane) Lane 4: HEK-293 cell lysate (20 ug/Lane) Lane 5: U-2 OS cell lysate (20 ug/Lane) Lane 6: K-562 cell lysate (20 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: HL-60 cell lysate (20 ug/Lane) Lane 9: Raji cell lysate (20 ug/Lane) Predicted band size: 107 kDa Observed band size: 107 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32367) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32367_rabbit-alpha-glucosidase-2-mab-psh01-53-278202415242675.jpg "Western Blot: alpha Glucosidase 2 Antibody (PSH01-53) [NBP3-32367] - Western blot analysis of alpha Glucosidase 2 on different lysates with Rabbit anti-alpha Glucosidase 2 antibody (NBP3-32367) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: LoVo cell lysate (20 ug/Lane) Lane 4: HEK-293 cell lysate (20 ug/Lane) Lane 5: U-2 OS cell lysate (20 ug/Lane) Lane 6: K-562 cell lysate (20 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: HL-60 cell lysate (20 ug/Lane) Lane 9: Raji cell lysate (20 ug/Lane) Predicted band size: 107 kDa Observed band size: 107 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32367) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Glucosidase 2 Antibody (PSH01-53) [NBP3-32367] - Immunofluorescence analysis of paraffin-embedded human thyroid tissue labeling alpha Glucosidase 2 with Rabbit anti-alpha Glucosidase 2 antibody (NBP3-32367) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32367, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32367_rabbit-alpha-glucosidase-2-mab-psh01-53-298202414333668.jpg)
![Immunohistochemistry: alpha Glucosidase 2 Antibody (PSH01-53) [NBP3-32367] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-alpha Glucosidase 2 antibody (NBP3-32367) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32367) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32367_rabbit-alpha-glucosidase-2-mab-psh01-53-29820241492672.jpg)
![Immunoprecipitation:alpha Glucosidase 2 AntibodyNBP3-29835] -Detection of human GANAB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GANAB antibody NBP3-29835 (lot NBP3-29835-1) used for IP at 6 µg per reaction. GANAB was also immunoprecipitated by rabbit anti-GANAB.. For blotting immunoprecipitated GANAB, A304-004A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29835_rabbit-alpha-glucosidase-2-pab-3012202412392129.jpg "Immunoprecipitation:alpha Glucosidase 2 AntibodyNBP3-29835] -Detection of human GANAB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GANAB antibody NBP3-29835 (lot NBP3-29835-1) used for IP at 6 µg per reaction. GANAB was also immunoprecipitated by rabbit anti-GANAB.. For blotting immunoprecipitated GANAB, A304-004A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: alpha Glucosidase 2 Antibody [H00023193-B01P] - Analysis of GANAB expression in A-431.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Western-Blot-H00023193-B01P-img0001.jpg "Western Blot: alpha Glucosidase 2 Antibody [H00023193-B01P] - Analysis of GANAB expression in A-431.")
![Western Blot: alpha Glucosidase 2 Antibody [H00023193-B01P] - Analysis of GANAB expression in transfected 293T cell line by GANAB polyclonal antibody. Lane1:GANAB transfected lysate(103.84 KDa). Lane2:Non-transfected lysate.](http://images.novusbio.com/fullsize/alpha-Glucosidase-2-Antibody-Western-Blot-H00023193-B01P-img0002.jpg)