![Western Blot: alpha Glucosidase 2 Antibody (PSH01-53) [NBP3-32367] - Western blot analysis of alpha Glucosidase 2 on different lysates with Rabbit anti-alpha Glucosidase 2 antibody (NBP3-32367) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: LoVo cell lysate (20 ug/Lane) Lane 4: HEK-293 cell lysate (20 ug/Lane) Lane 5: U-2 OS cell lysate (20 ug/Lane) Lane 6: K-562 cell lysate (20 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: HL-60 cell lysate (20 ug/Lane) Lane 9: Raji cell lysate (20 ug/Lane) Predicted band size: 107 kDa Observed band size: 107 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32367) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32367_rabbit-alpha-glucosidase-2-mab-psh01-53-278202415242675.jpg "Western Blot: alpha Glucosidase 2 Antibody (PSH01-53) [NBP3-32367] - Western blot analysis of alpha Glucosidase 2 on different lysates with Rabbit anti-alpha Glucosidase 2 antibody (NBP3-32367) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: LoVo cell lysate (20 ug/Lane) Lane 4: HEK-293 cell lysate (20 ug/Lane) Lane 5: U-2 OS cell lysate (20 ug/Lane) Lane 6: K-562 cell lysate (20 ug/Lane) Lane 7: Jurkat cell lysate (20 ug/Lane) Lane 8: HL-60 cell lysate (20 ug/Lane) Lane 9: Raji cell lysate (20 ug/Lane) Predicted band size: 107 kDa Observed band size: 107 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32367) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | PSH01-53 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within human alpha Glucosidase 2 aa 29-450. (Uniprot: Q14697) |
| Localization | Endoplasmic reticulum, Golgi apparatus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | GANAB |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 107 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | GANAB |
![Immunohistochemistry: alpha Glucosidase 2 Antibody (PSH01-53) [NBP3-32367] - Immunofluorescence analysis of paraffin-embedded human thyroid tissue labeling alpha Glucosidase 2 with Rabbit anti-alpha Glucosidase 2 antibody (NBP3-32367) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32367, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32367_rabbit-alpha-glucosidase-2-mab-psh01-53-298202414333668.jpg "Immunohistochemistry: alpha Glucosidase 2 Antibody (PSH01-53) [NBP3-32367] - Immunofluorescence analysis of paraffin-embedded human thyroid tissue labeling alpha Glucosidase 2 with Rabbit anti-alpha Glucosidase 2 antibody (NBP3-32367) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32367, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: alpha Glucosidase 2 Antibody (PSH01-53) [NBP3-32367] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-alpha Glucosidase 2 antibody (NBP3-32367) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32367) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32367_rabbit-alpha-glucosidase-2-mab-psh01-53-29820241492672.jpg "Immunohistochemistry: alpha Glucosidase 2 Antibody (PSH01-53) [NBP3-32367] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-alpha Glucosidase 2 antibody (NBP3-32367) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32367) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")