![Western Blot: Alpha Actinin 2 Antibody (ACTN2/3295) - Azide and BSA Free [NBP3-08788] - Western Blot of human Skeletal muscle tissue lysates using Alpha Actinin 2 Mouse Monoclonal Antibody (ACTN2/3295).](http://images.novusbio.com/fullsize/Alpha-Actinin-2-Antibody-ACTN2-3295-Azide-and-BSA-Free-Western-Blot-NBP3-08788-img0004.jpg "Western Blot: Alpha Actinin 2 Antibody (ACTN2/3295) - Azide and BSA Free [NBP3-08788] - Western Blot of human Skeletal muscle tissue lysates using Alpha Actinin 2 Mouse Monoclonal Antibody (ACTN2/3295).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, MA |
| Clone | ACTN2/3295 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07467). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | A recombinant fragment (aa557-692) of human Alpha Actinin 2 protein (exact sequence is proprietary) (Uniprot: P35609) |
| Localization | Cytoplasmic |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ACTN2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. |
| Theoretical MW | 103 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ACTN2 |
![Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody (ACTN2/3295) - Azide and BSA Free [NBP3-08788] - Formalin-fixed, paraffin-embedded human Cardiac Muscle stained with Alpha Actinin 2 Mouse Monoclonal Antibody (ACTN2/3295).](http://images.novusbio.com/fullsize/Alpha-Actinin-2-Antibody-ACTN2-3295-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08788-img0003.jpg "Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody (ACTN2/3295) - Azide and BSA Free [NBP3-08788] - Formalin-fixed, paraffin-embedded human Cardiac Muscle stained with Alpha Actinin 2 Mouse Monoclonal Antibody (ACTN2/3295).")
![Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody (ACTN2/3295) - Azide and BSA Free [NBP3-08788] - Formalin-fixed, paraffin-embedded human Skeletal Muscle stained with Alpha Actinin 2 Mouse Monoclonal Antibody (ACTN2/3295).](http://images.novusbio.com/fullsize/Alpha-Actinin-2-Antibody-ACTN2-3295-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08788-img0001.jpg "Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody (ACTN2/3295) - Azide and BSA Free [NBP3-08788] - Formalin-fixed, paraffin-embedded human Skeletal Muscle stained with Alpha Actinin 2 Mouse Monoclonal Antibody (ACTN2/3295).")
![Protein Array: Alpha Actinin 2 Antibody (ACTN2/3295) - Azide and BSA Free [NBP3-08788] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Alpha Actinin 2 Mouse Monoclonal Antibody (ACTN2/3295).](http://images.novusbio.com/fullsize/Alpha-Actinin-2-Antibody-ACTN2-3295-Azide-and-BSA-Free-Protein-Array-NBP3-08788-img0005.jpg "Protein Array: Alpha Actinin 2 Antibody (ACTN2/3295) - Azide and BSA Free [NBP3-08788] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Alpha Actinin 2 Mouse Monoclonal Antibody (ACTN2/3295).")
