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Alkaline Phosphatase/ALPP/ALPI Antibody [Unconjugated]

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Western blot shows lysates of human placenta tissue and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Alkaline Phosphatase/ALPP/ALPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5905) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Alkaline Phosphatase/ALPP/ALPI at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human placenta tissue and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Alkaline Phosphatase/ALPP/ALPI Antigen ...read more
Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Alkaline Phosphatase/ALPP/ALPI at approximately 76 kDa (as indicated) using 10 µg/mL of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IP
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5905-SP
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Alkaline Phosphatase/ALPP/ALPI Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Alkaline Phosphatase/ALPP
Ile23-Asp506
Accession # P05187
Specificity
Detects recombinant human Alkaline Phosphatase Placental Type/ALPP and recombinant human Alkaline Phosphatase Intestinal Type/ALPI in direct ELISAs. Detects human ALPP and ALPI but not Alkaline Phosphatase Liver Type/ALPL in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ALPP
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF5905 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Alkaline Phosphatase/ALPP/ALPI Antibody [Unconjugated]

  • Alkaline phosphatase Regan isozyme
  • alkaline phosphatase, placental type
  • Alkaline Phosphatase/ALPP/ALPI
  • alkaline phosphomonoesterase
  • ALP
  • ALPI
  • EC 3.1.3.1
  • glycerophosphatase
  • IAP
  • Intestinal alkaline phosphatase
  • intestinal-type alkaline phosphatase
  • PALP
  • Placental alkaline phosphatase 1
  • PLAP
  • PLAP-1

Background

ALPP (Alkaline phosphatase placental type; also SEAP, PALP-1 and ALP) is a 66-68 kDa glycoprotein member of the alkaline phosphatase family of molecules. It is principally expressed by 2nd and 3rd trimester synctiotrophoblasts and tumor cells, and represents one of four alkaline phosphatase isozymes. Experimentally, it cleaves phosphate monoesters into alcohol and phosphate. Human ALPP is synthesized as a 535 amino acid (aa) preproprecursor. It contains a 22 aa signal sequence, a 484 mature region (aa 23-506), and a 29 aa C-terminal propeptide that is cleaved to generate a GPI linkage at Asp506. The ALPP gene is highly allelic and may generate three potential splice variants that involve the signal sequence. The ALPP enzyme utilizes one Mg and two Zn ions, and functions as either a homodimer, or a heterodimer with the related Intestinal (ALPI) and Germ cell (GCAP) family members on the cell surface. ALPP is reported to oligomerize under certain conditions. Over aa 23-506, human ALPP shares 96% and 77% aa identity with chimpazeee ALPP and mouse embryonic-type alkaline phosphatase, respectively. It also shares 88% and 98% aa identity with IAP and GCAP, respectively. Over aa 23-506, human ALPP shares 86% aa identity with human ALPI.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Alkaline
Phosphatase/ALPP/ALPI ...
AF5905
Alkaline Phosphatase/ALPP/ALPI Antibody [Unconjugated]
Species: Hu
Applications: WB, Simple Western, IP

Publications for Alkaline Phosphatase/ALPP/ALPI Antibody (AF5905)(1)

We have publications tested in 1 confirmed species: Chicken.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol ALPP
Uniprot