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ALDH2 Antibody (1007902) [Unconjugated]

Images

 
Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat ALDH2 Monoclonal Antibody (Catalog # MAB10168) followed by ...read more
ALDH2 was detected in immersion fixed SK-BR-3 human breast cancer cell line (positive staining) and MCF-7 human breast cancer cell line (negative staining) using Mouse Anti-Human/Mouse/Rat ALDH2 Monoclonal Antibody ...read more
ALDH2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat ALDH2 Monoclonal Antibody (Catalog # MAB10168) at 0.5 µg/mL for 1 hour at room temperature ...read more
Simple Western lane view shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue, loaded at 0.2 mg/mL. A specific band was detected for ALDH2 at approximately 55 kDa (as indicated) using 20 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clone
1007902
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price
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ALDH2 Antibody (1007902) [Unconjugated] Summary

Immunogen
Synthetic peptide containing human ALDH2
Ser18-Ser517
Accession # P05091
Specificity
Detects human ALDH2 in direct ELISAs. Detects human, mouse, and rat ALDH2 in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 0.5-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ALDH2 Antibody (1007902) [Unconjugated]

  • acetaldehyde dehydrogenase 2
  • aldehyde dehydrogenase 2 family (mitochondrial)
  • aldehyde dehydrogenase, mitochondrial
  • ALDH class 2
  • ALDH2
  • ALDH-E2
  • ALDHI
  • ALDM
  • EC 1.2.1
  • EC 1.2.1.3
  • liver mitochondrial ALDH
  • MGC1806
  • nucleus-encoded mitochondrial aldehyde dehydrogenase 2

Background

Aldehyde Dehydrogenase 2 (ALDH2) is a protein involved in the pathway which synthesizes acetate from ethanol.  It also functions as a protector against oxidative stress.  The ALDH2 isoform is localized to the mitochondrial matrix.  Genetic variation in ALDH2 in the East Asian population is associated with high instances of acute alcohol intoxication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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