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Aldehyde dehydrogenase 5 Antibody

Images

 
Western Blot: Aldehyde dehydrogenase 5 Antibody [NBP1-90192] - Analysis in human cell line RH-30.
Immunocytochemistry/ Immunofluorescence: Aldehyde dehydrogenase 5 Antibody [NBP1-90192] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.
Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP1-90192] - Staining of human tonsil shows weak granular cytoplasmic positivity in non-germinal center cells.
Western Blot: Aldehyde dehydrogenase 5 Antibody [NBP1-90192] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma ...read more
Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP1-90192] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes and bile duct cells.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP1-90192] - Analysis in human liver and tonsil tissues using NBP1-90192 antibody. Corresponding ALDH1B1 RNA-seq data are ...read more
Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP1-90192] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP1-90192] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP1-90192] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Aldehyde dehydrogenase 5 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MLRFLAPRLLSLQGRTARYSSAAALPSPILNPDIPYNQLFINNEWQDAVSKKTFPTVNPTTGEVIGHV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALDH1B1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Aldehyde dehydrogenase 5 Protein (NBP1-90192PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Aldehyde dehydrogenase 5 Antibody

  • aldehyde dehydrogenase 1 family, member B1
  • Aldehyde dehydrogenase 5
  • Aldehyde dehydrogenase family 1 member B1
  • ALDH class 2
  • ALDH5aldehyde dehydrogenase X, mitochondrial
  • ALDHXacetaldehyde dehydrogenase 5
  • EC 1.2.1
  • EC 1.2.1.3
  • MGC2230
  • mitochondrial aldehyde dehydrogenase X

Background

Aldehyde dehydrogenase 5 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. This gene does not contain introns in the coding sequence. The variation of this locus may affect the development of alcohol-related problems. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ALDH1B1