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Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6)

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Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Analysis of ALDH1A1 expression in transfected 293T cell line by ALDH1A1 monoclonal antibody (M05), clone 1G6. Lane 1: ALDH1A1 ...read more
Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Analysis of monoclonal antibody to ALDH1A1 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ...read more
Immunoprecipitation: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Analysis of ALDH1A1 transfected lysate using anti-ALDH1A1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ...read more
ELISA: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Detection limit for recombinant GST tagged ALDH1A1 is 1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IP
Clone
1G6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
ALDH1A1 (AAH01505, 1 a.a. ~ 501 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSSSGTPDLPVLLTDLKIQYTKIFINNEWHDSVSGKKFPVFNPATEEELCQVEEGDKEDVDKAVKAARQAFQIGSPWRTMDASERGRLLYKLADLIERDRLLLATMESMNGGKLYSNAYLNDLAGCIKTLRYCAGWADKIQGRTIPIDGNFFTYTRHEPIGVCGQIIPWNFPLVMLIWKIGPALSCGNTVVVKPAEQTPLTALHVASLIKEAGFPPGVVNIVPGYGPTAGAAISSHMDIDKVAFTGSTEVGKLIKEAAGKSNLKRVTLELGGKSPCIVLADADLDNAVEFAHHGVFYHQGQCCIAASRIFVEESIYDEFVRRSVERAKKYILGNPLTPGVTQGPQIDKEQYDKILDLIESGKKEGAKLECGGGPWGNKGYFVQPTVFSNVTDEMRIAKEEIFGPVQQIMKFKSLDDVIKRANNTFYGLSAGVFTKDIDKAITISSALQAGTVWVNCYGVVSAQCPFGGFKMSGNGRELGEYGFHEYTEVKTVTVKISQKNS
Marker
Astrocyte Marker
Specificity
ALDH1A1 - aldehyde dehydrogenase 1 family, member A1 (1G6)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ALDH1A1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Reviewed Applications
Read 1 Review rated 1
using
H00000216-M05 in the following applications:

Publications
Read Publication using H00000216-M05.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6)

  • acetaldehyde dehydrogenase 1
  • ALDC
  • aldehyde dehydrogenase 1 family, member A1
  • aldehyde dehydrogenase 1, soluble
  • Aldehyde Dehydrogenase 1A1
  • Aldehyde Dehydrogenase 1-A1
  • Aldehyde dehydrogenase family 1 member A1
  • Aldehyde dehydrogenase, cytosolic
  • aldehyde dehydrogenase, liver cytosolic
  • ALDH class 1
  • ALDH1
  • ALDH11
  • ALDH1A1
  • ALDH-E1
  • ALHDII
  • EC 1.2.1
  • EC 1.2.1.36
  • MGC2318
  • PUMB1
  • RALDH 1
  • RALDH1
  • retinal dehydrogenase 1
  • retinaldehyde dehydrogenase 1

Background

This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (H00000216-M05)(1)


Review for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (H00000216-M05) (1) 11

Average Rating: 1
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00000216-M05:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
  1
reviewed by:
Verified Customer
WB Human 08/02/2023
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Summary

ApplicationWestern Blot
Sample TestedHuman pancreas/duct epithelioid carcinoma cell line - PANC1 Cells (ATCC # CRL-1469)
SpeciesHuman
LotG6141-1G6

Comments

Comments***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

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Verified Customer
08/02/2023
Application: WB
Species: Human

Bioinformatics

Gene Symbol ALDH1A1
Entrez
Uniprot