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Human AIF ELISA Kit (Chemiluminescence)

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ELISA: Human AIF ELISA Kit (Chemiluminescence) [NBP2-66673] - Samples were spiked with high concentrations of Human AIF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human AIF ELISA Kit (Chemiluminescence) [NBP2-66673] - Samples were spiked with high concentrations of Human AIF and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Human AIF ELISA Kit (Chemiluminescence) [NBP2-66673] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL

Order Details

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Human AIF ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Human AIF in samples. No significant cross-reactivity or interference between Human AIF and analogues was observed.
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 9.79%
Intra-Assay
CV% < 8.87%
Spike Recovery
85-104%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
AIFM1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Human AIF ELISA Kit (Chemiluminescence)

  • AIF
  • AIFapoptosis-inducing factor 1, mitochondrial
  • AIFM1
  • apoptosis-inducing factor, mitochondrion-associated, 1
  • COXPD6
  • MGC111425
  • PDCD8
  • PDCD8striatal apoptosis-inducing factor
  • programmed cell death 8 (apoptosis-inducing factor)
  • Programmed cell death protein 8

Background

AIF, or apoptosis-inducing factor, is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death, or apoptosis (1,2). AIF translocates to the nucleus when apoptosis is induced and causes mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. Apoptotic activity of AIF leads to chromatin condensation and DNA fragmentation (1). Similar to cytochrome c, AIF is a bifunctional protein with an oxidoreductase function and a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion (2,3,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

  Human AIF ELISA Kit (Chemiluminescence) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol AIFM1