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Human Adrenomedullin/ADM ELISA Kit (Colorimetric)

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ELISA: Human Adrenomedullin/ADM ELISA Kit (Colorimetric) [NBP2-78736] - Samples were spiked with high concentrations of Human Adrenomedullin/ADM and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Adrenomedullin/ADM ELISA Kit (Colorimetric) [NBP2-78736] - Samples were spiked with high concentrations of Human Adrenomedullin/ADM and diluted with Reference Standard & Sample Diluent to produce samples ...read more
ELISA: Human Adrenomedullin/ADM ELISA Kit (Colorimetric) [NBP2-78736] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL

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Human Adrenomedullin/ADM ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human ADM in samples. No significant cross-reactivity or interference between Human ADM and analogues was observed.
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.99%
Intra-Assay
CV% < 5.19%
Spike Recovery
92-112%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ADM

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Adrenomedullin/ADM ELISA Kit (Colorimetric)

  • ADM
  • Adrenomedullin
  • AMpreproadrenomedullin

Background

Adrenomedullin is a member of the calcitonin family of peptides and functions as a vasodilator. Adrenomedullin, a hypotensive peptide, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene related peptide. Human adrenomedullin mRNA was found to be highly expressed in several tissues. Adrenomedullain is secreted from a number of tissues including the adrenal medulla, endothelial and vascular smooth muscle cells. It has been implicated in a number of diseases including cardiovascular and renal disorders, sepsis, inflammation, diabetes and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Contact Information

Product PDFs

  SDS
  Human Adrenomedullin/ADM ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol ADM
Uniprot